维氏气单胞菌感染胭脂鱼的组织病理学研究  

Histopathology of Chinese Sucker (Myxocyprinus asiaticus) Infected with Virulent Aeromonas veronii

作　　者：李芳[1] 刘小红[1] 黄静[1] 张耀光[1] 王志坚[1] LI Fang;LIU Xiaohong;HUANG Jing;ZHANG Yaoguang;WANG Zhijian(College of Fisheries,Southwest University/Key Laboratory of Freshwater Fish Reproduction and Development,Ministry of Education/Key Laboratory of Aquatic Science of Chongqing,Chongqing 400715,China)

机构地区：[1]西南大学水产学院/淡水鱼类资源与生殖发育教育部重点实验室/水产科学重庆市重点实验室,重庆400715

出　　处：《西南大学学报（自然科学版）》2023年第2期86-95,共10页Journal of Southwest University(Natural Science Edition)

基　　金：国家自然科学基金青年基金项目(32202971);重庆市科技局技术创新与应用发展项目(4322200073)。

摘　　要：为研究维氏气单胞菌(Aeromonas veronii)感染胭脂鱼的组织病理特征,通过人工感染获得胭脂鱼肾(中肾、头肾)、脾脏、肝脏和肠道病理组织,结合组织学、组织化学以及透射电镜技术对组织样本进行观察,结果显示:维氏气单胞菌感染后,组织显微结构层面出现了出血、溶血、肝细胞空泡化、黑色素巨噬细胞中心(MMC)响应和淋巴细胞浸润等现象;超微结构层面显示:中肾肾小球过滤装置变形,头肾中巨噬细胞功能活跃,脾脏网状细胞和内皮细胞肿胀坏死,肝脏糖原消失,线粒体肿胀以及狄氏间隙结构遭到破坏;肠道组化染色结果显示:虽然肠道组织损伤严重,但其黏液细胞类型和数量并未发生显著变化.In order to study the hispathological characteristics of Aeromonas veronii(vAv) infected Chinese sucker(Myxocyprinus asiaticus), the pathological tissues of trunk kidney, head kidney, spleen, liver and intestine of vAv infected Chinese sucker were observed by histology, histochemistry and TEM technique. The results of histopathological examination revealed that the phenomena of hemorrhage, hemolysis, hepatocytes vacuolation, MMCs response and lymphocyte infiltration were observed in the microstructure of tissues. The ultrastructure level showed the malformation of glomerulus filtration device in trunk kidney, macrophages activated in head kidney, reticular cells and endothelial cells edema necrosis in spleen, the disappearing of glycogen, mitochondria swelling and the degeneration of the diss space in liver. The results of intestinal histochemical staining showed that although the intestinal tissue was seriously damaged, the type and number of mucus cells did not change significantly.

关 键 词：维氏气单胞菌 胭脂鱼 黑色素巨噬细胞中心 黏液细胞 

分 类 号：Q95-3[生物学—动物学]