基于水稻矮秆长粒CSSL-Z688的QTL鉴定及SSSLs培育  

Identification of QTL Based on a Dwarf and Long-Large Grain Rice CSSL-Z688 and Development of SSSLs

作　　者：李苗苗 李儒香 秦鱼河 邓岢莉 余金琎 徐光益 向思茜 杨正林[1] 桑贤春[1] 凌英华[1] 何光华[1] 赵芳明[1] LI Miaomiao;LI Ruxiang;QIN Yuhe;DENG Keli;YU Jinjin;XU Guangyi;XIANG Siqian;YANG Zhenglin;SANG Xianchun;LING Yinghua;HE Guanghua;ZHAO Fangming(Rice Research Institute,Southwest University/Academy of Agricultural Sciences,Chongqing 400715,China;Luzhou Taifeng Seed Company,Luzhou Sichuan 646009,China)

机构地区：[1]西南大学水稻研究所/农业科学研究院,重庆400715 [2]泸州泰丰种业有限公司,四川泸州646009

出　　处：《西南大学学报（自然科学版）》2023年第1期33-44,共12页Journal of Southwest University(Natural Science Edition)

基　　金：重庆市重点实验室开放基金项目(ZYZDSYSKFXM202102);重庆市水稻分子设计创新群体项目(cstc2021jcyj-cxttX0004).

摘　　要：水稻株高、粒型等性状是由多基因控制的数量性状,遗传基础复杂.水稻染色体片段代换系(CSSL)是研究复杂性状的理想遗传材料,然而目标基因的水稻单片段代换系(SSSL)的培育则需要多年多代逐步完成.以4代换片段的水稻矮秆长大粒CSSL-Z688为研究材料,鉴定出7个株高及粒型性状的数量性状基因座(quantitative trait locus,QTL);进一步培育了目标QTL的5个纯合单片段代换系和5个杂合单片段代换系,其中6个QTLs(qPH1-1,qPH1-2,qGL1,qGL12,qRLW1和qGWT1)能够被纯合单片段代换系所验证.还鉴定出11个新QTLs,分别为qPH2,qGL1-2,qGL2,qGW1,qRLW1-2,qRLW2,qRLW12,qGWT1-2,qGWT2,qGWT6和qGWT12,其中7个QTLs尚未被报道.QTL加性和显性效应表明:水稻株高、粒长、千粒质量等表型同时受到多个QTL加性和显性效应的共同影响,如来自供体西恢18的qph1-1,qPH1-2,qPH2的加性效应和qPH1-1/qph1-1及qPH1-2/qph1-2和qPH2/qph2的显性效应共同影响株高的遗传;来自西恢18的qGL1,qgl1-2,qGL2和qGL12的加性效应及qGL1/qgl1的显性效应共同影响水稻粒长的遗传;来自西恢18的qGWT1,qGWT1-2,qGWT2,qGWT6和qGWT12的加性效应及qGWT1/qgwt1,qGWT1-2/qgwt1-2,qGWT6/qgwt6,qGWT2/qgwt2和qGWT12/qgwt12的显性效应共同影响千粒质量的遗传.这些结果表明单片段代换系的构建可有效地将遗传复杂的数量性状分解为单位点进行研究,为以单片段代换系为平台的水稻分子设计育种提供了重要遗传信息.Plant height and grain size are quantitative traits in rice,which is controlled by multiple genes,and their genetic basis is complex.Rice chromosome segment substitution line(CSSL)is an ideal genetic material for studying complex traits.However,development of single segment substitution lines(SSSLs)needs many years and many generations to complete.In this study,seven QTLs of related traits(plant height,grain length,length width ratio,1000 grain weight)were identified by using a 4 generation of rice dwarf and long grain substitution segments line CSSL Z688.Five homozygous SSSLs and five heterozygous SSSLs for the target QTLs were further developed.Among the target QTLs,6 of them(qPH1-1,qPH1-2,qGL1,qGL12,qRLW1 and qGWT1)could be verified by 5 homozygous SSSLs.In addition,11 new QTLs(qPH2,qGL1-2,qGL2,qGW1,qRLW1-2,qRLW2,qRLW12,qGWT1-2,qGWT2,qGWT6 and qGWT12)were identified by 5 single segment substitution lines,among them,7 QTLs were not reported previously.Additive and dominant effects of QTL showed that the phenotypes of plant height,grain length and 1000-grain weight were all influenced by the additive and dominant effects of many QTLs.For example,the additive effects of qph1-1,qPH1-2,qPH2 from Xihui 18 and the dominant effects of qPH1-1/qph1-1,qPH1-2/qph1-2 and qPH2/qph2 in combination affected the inheritance of plant height in rice.The additive effect of qGL1,qgl1-2,qGL2 and qGL12,as well as the dominant effect of qGL1/qgl1 simultaneously influenced the inheritance of grain length.Similarly,the additive effects of qGWT1,qGWT1-2,qGWT2,qGWT6 and qGWT12,together with the dominant effects of qGWT1/qgwt1,qGWT1-2/qgwt1-2,qGWT6/qgwt6,qGWT2/qgwt2 and qGWT12/qgwt12 had combined effects on the inheritance of 1000 grain weight.These results suggested that development of SSSLs can effectively dissect the quantitative traits with complex inheritance into single QTL/gene for research,also can provide important genetic information for rice molecular breeding by design based on the platform of single segment substitution lines

关 键 词：水稻 染色体片段代换系(CSSL) 粒型 株高 QTL定位 

分 类 号：S511[农业科学—作物学]