陆地棉RIL群体产量与纤维品质性状QTL定位  

QTL Mapping of Yield and Fiber Quality Traits in an Upland Cotton RIL Population

作　　者：黄莎 张正圣[1] 王文文 刘大军[1] HUANG Sha;ZHANG Zhengsheng;WANG Wenwen;LIU Dajun(School of Agronomy and Biotechnology,Southwest University,Chongqing 400715,China)

机构地区：[1]西南大学农学与生物科技学院,重庆400715

出　　处：《西南大学学报（自然科学版）》2023年第1期55-65,共11页Journal of Southwest University(Natural Science Edition)

基　　金：科技部国家重点研发计划资助项目(2016YED0100203-2).

摘　　要：棉花是世界首要的天然纤维作物,同时也是重要的蛋白和油料作物.该研究构建了一个包含184个单株的(渝棉1号×超早3号)重组近交系群体.利用SSR和SLAF-seq SNP分子标记共同构建高密度遗传图谱,结合多种环境鉴定产量和纤维品质性状表型,定位棉花产量和纤维品质性状的QTL.研究结果表明:①对8020个SSR与SNP标记进行遗传连锁分析,构建的遗传图谱共2945个位点(41 SSR和2904 SNP),遗传长度为4650.71 cM,覆盖陆地棉基因组总长的98.30%;②共定位到76个QTL,包括35个产量性状QTL,41个纤维品质性状QTL,LOD值分布在2.50~7.76之间,解释表型变异率为6.4%~23.4%;③10个QTL在两个及以上环境被检测到,为环境稳定QTL.Cotton is the leading natural fiber crop,and also an important protein crop and oil crop in the world.A RIL population consisting of 184 lines(Yumian 1×Chaozao 3)was constructed in this study.The SNP markers obtained by SLAF-seq and polymorphic SSR markers were used to construct a high-density genetic map.The phenotypic data of yield and fiber quality traits detected in multiple environments were used to locate QTL for yield and fiber quality traits.The results are as follows:①Linkage analysis was conducted on 8020 markers,and a genetic map containing 2945(41 SSR and 2904 SNP)loci were finally constructed.The genetic length of the constructed map was 4650.71 cM,and covered 98.30%of the upland cotton genome.②Totally,35 QTLs for yield traits and 41 QTLs for fiber quality traits were detected,which explained 6.4%to 23.4%of the phenotypic variation,and their LOD value ranged from 2.50 to 7.76.③Ten QTLs were detected in two or more environments,which were environment-stable QTL.

关 键 词：陆地棉 简化基因组测序 产量 纤维品质 数量性状位点 遗传图谱 

分 类 号：S562[农业科学—作物学]