补体C5a受体1拮抗剂对小鼠脑缺血/再灌注的保护作用  

Protective effect of complement C5a receptor 1 antagonist on cerebral ischemia-reperfusion in mice

作　　者：石艳超[1] 韩晋 李强 孟虹媛[1] SHI Yanchao;HAN Jin;LI Qiang;MENG Hongyuan(Department of Neurology,Port hospital of Tianjin,Tianjin 300456,China)

机构地区：[1]天津港口医院神经内科,天津300456

出　　处：《中风与神经疾病杂志》2022年第12期1082-1085,共4页Journal of Apoplexy and Nervous Diseases

摘　　要：目的探讨补体C5a受体1(C5aR1)拮抗剂对小鼠脑缺血/再灌注(CIR)的保护作用。方法将小鼠随机分为假手术组、CIR组(模型组)和C5aR1拮抗剂(PMX53)组。造模前3 h、再灌注24 h及再灌注48 h,PMX53组给予腹腔注射PMX53,假手术组和模型组给予腹腔注射同体积生理盐水。再灌注72 h,使用改良Longa法对小鼠行神经功能缺损评分,TTC染色后计算缺血脑组织体积,干湿重法测缺血侧半球脑组织含水量,RT-PCR法检测缺血侧半球脑组织炎性因子mRNA的表达,Western blot法计算缺血侧半球脑组织ZO-1的相对表达量。结果脑再灌注72 h,与模型组相比,PMX53组小鼠神经功能缺损评分、脑含水量、脑缺血体积及炎性因子(IL-1β和TNF-α)的表达均降低(均P<0.05),ZO-1的表达升高(P<0.05)。结论C5aR1拮抗剂可改善CIR后神经功能,减小脑缺血体积,减轻脑水肿程度以及减轻CIR后炎性反应,对血脑屏障具有保护作用。Objective To investigate the protective effect of complement C5a receptor 1(C5aR1)antagonist on cerebral ischemia-reperfusion(CIR)in mice.Method Mice were randomly divided into sham operation group,cerebral ischemia-reperfusion group(model group)and C5aR1 antagonist(PMX53)group.At 3 h before,24 h after and 48 h after cerebral reperfusion timepoint,the PMX53 group was given with PMX53,the sham group and the model group were given same volume of saline by intraperitoneall injection.At 72 h after cerebral reperfusion timepoint,neurological deficits score of mice were evaluated by the modified Longa method,the infarcted brain volume was calculated after TTC staining,the cerebral tissue water content of the ischemic hemisphere was calculated by dry and wet weight method,the mRNA expression of inflammatory factors in the ischemic hemisphere were detected by real-time PCR,and the relative expression of ZO-1 in cerebral tissue of the ischemic hemisphere was calculated by Western blot.Results At 72 h after cerebral reperfusion,compared with the model group,neurological deficits function score,cerebral water content,cerebral infarction volume and inflammatory cytokines(IL-1β,TNF-α)were significantly decreased in the PMX53 group(all P<0.05),ZO-1 expression was significantly higher in the PMX53 group(P<0.05).Conclusion C5aR1 antagonist can improve the neurological function score after CIR,reduce the volume of cerebral infarction,reduce the degree of cerebral edema and inflammatory response,and has a protective effect on the blood-brain barrier.

关 键 词：脑缺血/再灌注 炎性反应 C5a受体1拮抗剂 脑保护 

分 类 号：R743.3[医药卫生—神经病学与精神病学]