水稻半育突变体sfp10的生理特征分析及基因定位  

Physiological characteristics analysis and gene mapping of a semi-sterility plant mutant sfp10 in rice(Oryza sativa L.)

作　　者：李秋平 张春龙 杨宏 王拓 李娟[1] 金寿林[1] 黄大军[1] 李丹丹 文建成[1] LI Qiu-Ping;ZHANG Chun-Long;YANG Hong;WANG Tuo;LI Juan;JIN Shou-Lin;HUANG Da-Jun;LI Dan-Dan;WEN Jian-Cheng(Rice Research Institute of Yunnan Agricultural University,Kunming 650201,Yunnan,China;Key Laboratory of Crop Production and Intelligent Agriculture of Yunnan Province,Kunming 650201,Yunnan,China)

机构地区：[1]云南农业大学稻作研究所,云南昆明650201 [2]云南省作物生产与智慧农业重点实验室,云南昆明650201

出　　处：《作物学报》2023年第3期634-646,共13页Acta Agronomica Sinica

基　　金：云南省重大科技专项(202102AE090017);云南省基础研究计划面上项目(202201AT070263);云南省教育厅科学研究基金项目(2022J0280);云南省作物生产与智慧农业重点实验室开放基金课题(20210101)资助。

摘　　要：以水稻花粉半育性突变体lsm与籼稻93-11构建的高世代回交导入系semi-fertility plant 10(sfp10)为研究对象,与野生型93-11相比,突变体在在株高、叶长、叶宽、分蘖数、花粉数量等农艺性状上均未发现显著差异,但花粉育性却显著下降。花粉镜检及花粉发育后期的扫描电镜观察结果显示,sfp10突变体部分花粉在发育后期淀粉积累减少并最终败育。花粉发育相关生理指标检测结果表明,突变体花药中脯氨酸和淀粉的含量显著下降;蔗糖在突变体穗部上游组织(源叶、库叶、茎)中积累量显著增加,但穗部含量却明显减少,说明蔗糖到穗部的运输过程受到影响。遗传分析表明,sfp10突变体性状受1对隐性核基因控制,基因初定位将突变位点定位于水稻10号染色体RM25389和RM25404之间的398 kb区间内,该区间包含3个与蔗糖转运相关的基因和1个与淀粉合成相关的基因。本研究为进一步开展花粉半育性调控基因的精细定位、基因功能及调控机制的深入研究奠定基础。In this study,the advanced backcross population of semi-fertility plant 10(sfp10)mutation constructed by rice pollen semi-fertile mutant lsm and indica rice cultivar 93-11(wild-type,WT)was used as the research subjects.Compared to the WT,there were no significant differences in plant height,leaf length,leaf width,tiller number,pollen number and other agronomic traits,but pollen fertility reduced significantly.Pollen microscopy and scanning electron microscopy(SEM)at the late stage of pollen development showed that starch accumulation in some pollen of sfp10 decreased observably,eventually led to pollen abortion.Physiological indexes related pollen development revealed that the contents of proline and starch in pollen of the sfp10 mutant decreased significantly.Sucrose accumulation generally increased in the upstream tissues(source leaf,sink leaf,and stem)of panicle,but sucrose content decreased significantly in panicle,indicating that transport from sucrose to panicle was affected.Genetic analysis indicated that the sfp10 phenotype was controlled by a pair of recessive nuclear genes.Gene preliminary mapping located the mutant site into a 398 kb interval between RM25389 and RM25404 on rice chromosome 10,which contained 3 genes related to sucrose transport and 1 gene related to starch synthesis.This study laid a foundation for further research on fine mapping,gene function,and regulation mechanism of pollen semi-fertility regulation genes.

关 键 词：水稻 半育性植株 sfp10 基因定位 淀粉积累 

分 类 号：S511[农业科学—作物学]