猪真皮深层组织错位损伤对皮肤纤维化的影响  

Effect of deep dermal tissue dislocation injury on skin fibrosis in pig

作　　者：余小平[1] 刘英开[2] 马先 唐佳俊[2] 牛轶雯[2] 周军利[1] 陆树良[2] Yu Xiaoping;Liu Yingkai;Ma Xian;Tang Jiajun;Niu Yiwen;Zhou Junli;Lu Shuliang(Department of Burns,Gansu Provincial Hospital,Lanzhou 730010,China;Wound Repair Center,Ruijin Hospital,Shanghai Jiao Tong University School of Medicine,Shanghai Wound Repair Research Center,Shanghai 200025,China)

机构地区：[1]甘肃省人民医院烧伤科,兰州730010 [2]上海交通大学医学院附属瑞金医院创面修复中心,上海市创面修复研究中心,上海200025

出　　处：《中华烧伤与创面修复杂志》2022年第11期1057-1065,共9页Chinese Journal of Burns And Wounds

基　　金：国家自然科学基金面上项目(81460294,81101433,81071566);甘肃省自然科学基金(21JR7RA609,21JR7RA674)。

摘　　要：目的探讨猪真皮深层组织错位损伤对皮肤纤维化的影响,以期为烧伤瘢痕治疗提供一定的理论依据。方法采用实验研究方法。取6只2个月龄雌性杜洛克猪,将背部右侧切取中厚皮片和真皮深层组织片并原位回植的15个术区纳入真皮原位回植组,将背部左侧切取中厚皮片和真皮深层组织片并将真皮深层组织片放在脂肪层下的15个术区纳入真皮错位组。观察2组伤后7、14、21 d术区毛发生长情况及伤后14 d横断面结构。伤后7、14、21 d,测量并比较2组术区皮肤厚度(表皮到脂肪上缘的距离)、真皮厚度(表皮下缘到脂肪上缘的距离,不包括真皮和脂肪间的纤维组织厚度)、真皮-脂肪界面(真皮原位回植组为真皮深层下缘至脂肪上缘、真皮错位组为真皮浅层下缘至脂肪上缘)的纤维组织厚度,测量真皮原位回植组术区真皮切割界面(真皮浅层下缘至真皮深层上缘)纤维组织厚度并与真皮-脂肪界面纤维组织厚度进行比较;行天狼猩红染色,观测并比较2组术区真皮-脂肪界面及真皮原位回植组术区真皮切割界面和真皮-脂肪界面Ⅰ、Ⅲ型胶原蛋白含量;行免疫组织化学染色观察2组术区增殖细胞核抗原(PCNA)、转化生长因子β_(1)(TGF-β_(1))、成纤维细胞生长因子2(FGF-2)、肝细胞生长因子(HGF)的阳性表达。样本数均为6。对数据行独立样本t检验。结果伤后7、14、21 d,真皮原位回植组术区毛发均较真皮错位组浓密。伤后14 d,真皮错位组术区皮肤横断面呈“三明治”样结构,真皮原位回植组术区皮肤横断面结构正常。伤后7、14、21 d,真皮错位组术区皮肤厚度分别为(4234±186)、(4688±360)、(4548±360)μm,与真皮原位回植组的(4425±156)、(4714±141)、(4310±473)μm均相近(P>0.05);真皮错位组术区真皮厚度均较真皮原位回植组明显变薄(t值分别为-9.73、-15.85、-15.41,P<0.01);真皮错位组术区真皮-脂肪界面纤维组织Objective To explore the effect of deep dermal tissue dislocation injury on skin fibrosis in pig,in order to provide some theoretical basis for burn scar treatment.Methods The experimental research method was applied.Six 2-month-old female Duroc pigs were taken.Fifteen operative areas on the right dorsum of pigs on which medium-thick skin grafts and deep dermal tissue slices were cut and re-implanted were included into dermal in situ reimplantation group,and fifteen operative areas on the left dorsum of pigs on which medium-thick skin grafts and deep dermal tissue slices were cut and the deep dermal tissue slice was placed under the fat layer were included into the dermal dislocation group.The hair growth in the operative areas on post-injury day(PID)7,14,and 21 and the cross-sectional structure on PID 14 were observed in the two groups.On PID 7,14,and 21,the skin thickness(the distance from the epidermis to the upper edge of the fat),the dermal thickness(the distance from the lower edge of the epidermis to the upper edge of the fat,excluding the fibrotic tissue thickness between the dermis and the fat),and the fibrosis tissue thickness of the dermis-fat interface(from the lower edge of the deep dermis to the upper edge of the fat in dermal in situ reimplantation group and from the lower edge of the superficial dermis to the upper edge of the fat in dermal dislocation group)in the operative areas were measured and compared between the two groups;the fibrotic tissue thickness at the dermal cutting interface(from the lower edge of the superficial dermis to the upper edge of the deep dermis)in the operative areas in dermal in situ reimplantation group was measured and compared with the fibrotic tissue thickness at the dermal-fat interface.Sirius red staining was performed to observe and compare the typeⅠandⅢcollagen content in the dermal-fat interface in the operative areas between the 2 groups and between the dermal cutting interface and dermal-fat interface in the operative areas in dermal in situ reimplantati

关 键 词：皮肤 瘢痕 纤维化 脂肪组织 真皮深层组织 切割伤 

分 类 号：R622[医药卫生—整形外科]