结核分枝杆菌McSpoligotyping基因分型在宜昌地区耐多药结核病中的应用研究  

Application of Mycobacterium tuberculosis genotyping by McSpoligotyping in MDR-TB in Yichang

作　　者：何建 胡晓红 周萍 胡芳 刘鹏 周小妮 HE Jian;HU Xiao-hong;ZHOU Ping;HU Fang;LIU Peng;ZHOU Xiao-ni(Department of Tuberculosis Disease,The Third People's Hospital of Yichang,443003,China)

机构地区：[1]宜昌市第三人民医院结核科,443003 [2]宜昌市第三人民医院检验科,443003 [3]宜昌市疾病控制中心结防所,443005

出　　处：《传染病信息》2022年第6期538-543,共6页Infectious Disease Information

基　　金：宜昌市医疗卫生科研项目(A19-301-48)。

摘　　要：目的运用熔解曲线间隔区寡核苷酸分型(melting curve spacer oligonucleotide typing,McSpoligotyping)方法对宜昌地区耐多药结核分枝杆菌进行基因分型,以评价其在结核病流行病学调查中的应用价值。方法选择2019年1月—2020年12月耐多药菌株77株作为实验组,并以异烟肼和利福平敏感菌株110株作为对照组,运用Mc Spoligotyping方法进行基因分型,将分型结果与Spol DB4.0数据库进行比对分析。结果187株菌株中北京基因型116株(62.03%),非北京基因型71株(37.97%)。对照组110株中北京基因型60株(54.55%),其中数量最多的1组国际型别编码(spoligotype inter-national type,SIT)为1的北京基因型共44株(40.00%);非北京基因型50株(45.45%),以T族、Manu族常见。实验组77株中北京基因型56株(72.73%),其中数量最多的1组为SIT为1的北京基因型共44株(57.14%),非北京基因型21株(27.27%),以T族、Manu2族常见。实验组北京基因型菌株所占比例与对照组差异有统计学意义。实验组成簇率(79.22%)与对照组成簇率(79.09%)差异无统计学意义。结论北京基因型菌株是宜昌地区结核病的主要流行菌株,在耐多药结核病患者中北京基因型菌株更为流行。耐多药结核菌株有一定的遗传多态性。Mc Spoligotyping方法操作简单、快速、对于结核病分子流行病学研究具有重要意义。Objective To study the genotype multidrug-resistant Mycobacterium tuberculosis by melting curve spacer oligonucleotide method(McSpoligotyping)and evaluate its value in epidemiological investigation of tuberculosis in Yichang.Methods Seventy-seven MDR-TB strains were selected from January 2019 to December 2020 as the experimental,and 110HR-sensitive strains were selected as the control.Genotyping was performed using melting curve spacer oligonucleotide typing(Mc Spoligotyping),and the results were compared with the SpolDB4.0 database.Results Of the 187 strains,116(62.03%)were Beijing genotype and 71(37.97%)were non-Beijing genotype.Among the 110 strains in the control,60(54.55%)had Beijing genotype,of which the largest group was 44(40.00%)with Beijing genotype with SIT of 1;50(45.45%)had non-Beijing genotype,with the T and Manu groups being common.Among the 77 strains in the experimental group,56(72.73%)were Beijing genotype,of which the largest group was 44(57.14%)Beijing genotype with SIT of 1 and 21(27.27%)non-Beijing strains,which were common in T and Manu2families.The proportion of Beijing genotype strains in the experimental group was significantly different from the control(P<0.05).There was no significant difference in the cluster rate between the experimental(79.22%)and the control group(79.09%)(P>0.05).Conclusions The Beijing genotype strains is the main prevalent strain of tuberculosis in the Yichangand tis more prevalent in MDR-TB patients.MDR-TB strains have some genetic polymorphism.The McSpoligotyping method is simple,fast and it is great significance for the molecular epidemiological studies of tuberculosis.

关 键 词：结核分枝杆菌 北京基因型 熔解曲线 间隔区寡核苷酸分型法 分子流行病学 耐多药结核病 异烟肼 利福平 成簇炎 

分 类 号：R52[医药卫生—内科学]