洗手产品除菌效果评价的离体试验方法研究  

In vitro test method for evaluation of germicidal efficacy ofhand washing products

作　　者：孙廷丽 李彩玲 陈静 叶一均 林小涵 杨义熙 谢小保 SUN Tingli;LI Cailing;CHEN Jing;YE Yijun;LIN Xiaohan;YANG Yixi;XIE Xiaobao(Key Laboratory of Agricultural Microbiomics and Precision Application(MARA),Guangdong Provincial Key Laboratory of Microbial Culture Collection and Application,Key Laboratory of Agricultural Microbiome(MARA),State Key Laboratory of Applied Microbiology Southern China,Guangdong Detection Center of Microbiology,Institute of Microbiology,Guangdong Academy of Sciences,Guangzhou 510070)

机构地区：[1]广东省科学院微生物研究所,广东省微生物分析检测中心,华南应用微生物国家重点实验室/农业农村部农业微生物组学与精准应用重点实验室/农业农村部农业微生物组学重点实验室/广东省菌种保藏与应用重点实验室,广东广州510070

出　　处：《工业微生物》2022年第6期10-15,共6页Industrial Microbiology

基　　金：国家自然科学基金(项目号:32102647)。

摘　　要：本文以金黄色葡萄球菌和大肠杆菌为实验菌种,以仿真皮为实验载体,以四种洗手产品为试验对象,验证了仿真皮离体试验与在体试验的相关性,建立了一种洗手产品离体洗手除菌效果评价的试验方法,并建立以统计学为基础的评价方法。结果显示,离体仿真皮为试验载体时,与在体试验相关性好;离体实验方法适用于多种洗手产品,具有良好的可重复性,是一种简单可行的洗手产品除菌效果的评价方法,具有较好的应用价值。In this paper,using Staphylococcus aureus and Escherichia coli as the test strains,simulated skin as experimental carrier,and four kinds of hand washing products as experimental objects,the correlation between in vitro test and in vivo test of simulated skin was verified.A test method for evaluating the germicidal efficacy of hand washing products in vitro was established.Meanwhile,an evaluation method based on statistics was also established.The results showed that the in vitro test with simulated skin as experimental carrier had good correlation with the in vivo test.And the in vitro test method was suitable for many kinds of hand washing products and had good repeatability.It was a simple and feasible method to evaluate the germicidal efficacy of hand washing products,and had good application value.

关 键 词：离体洗手模型 洗手产品 除菌效果 试验方法 

分 类 号：R187[医药卫生—流行病学]