白术提取工艺及抗氧化性能研究  

Extraction of atractylodis macrocephalae rhizoma and its antioxidant capacity

作　　者：宋晨 闫璐 Song Chen;Yan Lu(Handan Ruichuan Technology Corporation Ltd.,Handan 056000,China)

机构地区：[1]邯郸睿川科技有限公司,河北邯郸056000

出　　处：《煤炭与化工》2022年第11期144-147,共4页Coal and Chemical Industry

摘　　要：为提高白术综合提取率,以蒸馏水为溶剂,采用直接蒸馏法收集白术水提物、挥发油、滤渣进一步醇提得白术醇提物。由正交实验法以黄酮得率为指标优化工艺参数:料液(水)比为1∶20(mL/g),蒸馏时间为5 h,料液(醇)比1∶20(mL/g),微波时间为4 min。此条件下,黄酮得率0.44%,多糖得率30.42%,水和醇总提取物得率35.21%。进一步,以最优工艺所得提取物进行抗自由基实验,结果表明,50μg/mL白术水提物对·OH清除率达21.96%,50 ug/mL白术醇提物清除率为18.89%;40 ug/mL白术水提物对DPPH·清除率达17.62%,40 ug/mL白术醇提物清除率为12.85%。In order to improve the comprehensive extraction rate of atractylodes macrocephala,the comprehensive extraction of atractylodis macrocephalae rhizomastarted with direct distillation usingdistilled water as solvent to give aqueous extracts and volatile oil.The residue went through further alcohol extraction to give ethanol extracts.orthogonal test was conducted with flavone yield as inspection target to obtain optimal extraction condition,namely solid/solvent(water)ratio of 1:20(mL/g),distillation time of 5 h,solid/solvent(ethanol)ratio of 1:20(mL/g),and microwave treatment time of 4 min.Such conditions yield 0.44%of flavone,30.42%of polysaccharide,and a total 35.21%yield from water and ethanol extraction.Further,the anti free radical experiment was carried out on the extract obtained by the optimal process.The results show that:a scavenging activity of 21.96%with OH free radical was observed at 50μg/mL ratio of aqueous extracts,and that of ethanol extracts of the same ratio was 18.89%.The optimal scavenging activity of 17.62%with DPPH free radical was observed at 40μg/mL ratio of aqueous extracts,with a scavenging activity of 12.85%at ethanol extracts of the same ratio.

关 键 词：白术 微波提取 水提物 醇提物 抗氧化 抗自由基 

分 类 号：TQ461[化学工程—制药化工]