金纳米棒调控碳量子点荧光探针的构建与分析  

Construction and Analysis of Fluorescent Probes for Carbon Quantum Dots Regulated by Gold Nanorods

作　　者：陆筑凤[1,2] 王红梅[2] 李加友[2] 程若冰 刘露 LU Zhufeng;WANG Hongmei;LI Jiayou;CHENG Ruobing;LIU Lu(Analysis and Testing Center,Chemical Sciences and Engineering,Jiaxing University,Jiaxing 314001,Zhejiang,China;College of Biological,Chemical Sciences and Engineering,Jiaxing University,Jiaxing 314001,Zhejiang,China)

机构地区：[1]嘉兴学院分析测试中心,浙江嘉兴314001 [2]嘉兴学院生物与化学工程学院,浙江嘉兴314001

出　　处：《实验室研究与探索》2022年第10期33-38,共6页Research and Exploration In Laboratory

基　　金：浙江省公益技术应用分析测试项目(LGC21B050010)。

摘　　要：以金纳米棒对碳量子点荧光发射行为的调控为基础,构建了高稳定性和选择性的新型复合纳米荧光探针。实验以柠檬酸铵和乳糖为原料一步水热法制备碳量子点,采用种子生长法以氯金酸、硼氢化钠和十六烷基三甲基溴化铵为原料两步合成不同长径比的金纳米棒,并对分析材料性能进行了表征。结果表明:碳量子点平均粒径6 nm,最大激发和发射波长为335和440 nm,荧光量子产率17.6%;不同长径比(R=8~15)的金纳米棒对碳量子点有不同程度的荧光猝灭,其中R=15的金纳米棒在508 nm处有最大紫外吸收,对碳量子点的荧光猝灭最强,调节体系盐浓度可实现荧光恢复,成功建立新型荧光探针,解决了碳量子点荧光性能易受其他物质干扰的问题,提高了探针的选择性。Based on the regulation of fluorescence emission behavior of carbon quantum dots by gold nanorods and combined with fluorescence analysis technology,a novel composite fluorescent probe with high stability and selectivity was constructed.In this experiment,carbon quantum dots were synthesized by one-step hydrothermal method using ammonium citrate and lactose as raw materials.Gold nanorods with different length-diameter ratio were prepared by seed growth method using chlorauric acid,sodium borohydride and cetyltrimethyl ammonium bromide as raw materials.The properties of the materials were characterized.The results show that the average particle size of carbon quantum dots is 6 nm,the maximum excitation and emission wavelengths are respectively 335 nm and 440 nm,fluorescence quantum yield is 17.6%.Gold nanorods with different length-diameter ratio were prepared.The length-diameter ratio was 8~15.Gold nanorods with different length-diameter ratio can quench the fluorescence of carbon quantum dots to different degrees.Among them,gold nanorods(R=15)with the maximum UV absorption at 508 nm have the strongest fluorescence quenching on carbon quantum dots.Fluorescence recovery can be achieved by adjusting the salt concentration of the system,and a new fluorescence probe can be successfully established.Thus,the problem that the fluorescence performance of carbon quantum dots is easily interfered by other substances is solved and the selectivity of the probe is improved.

关 键 词：碳量子点 金纳米棒 长径比 荧光探针 荧光猝灭 荧光恢复 

分 类 号：TB34[一般工业技术—材料科学与工程] G642.423[文化科学—高等教育学]