血管分泌因子Rspondin3调控Wntβ-catenin信号通路促进急性肺损伤组织修复的机制研究  

The Mechanism of Vascular Secretory Factor Rspondin3 Regulating Wntβ-catenin Signaling Pathway to Promote Tissue Repair in Acute Lung Injury

作　　者：李鹏 欧阳运萍 陈涛 赵博 杨小军[1] LI Peng;OUYANG Yunping;CHEN Tao(Tangshan Union Medical College Hospital,Hebei Tangshan 063000,China)

机构地区：[1]河北省唐山市协和医院,河北唐山063000

出　　处：《河北医学》2023年第1期37-41,共5页Hebei Medicine

基　　金：河北省医学科学研究课题,(编号:20201544)。

摘　　要：目的:探讨血管分泌因子Rspondin3通过wnt/β-catenin信号通路参与急性肺损伤大鼠模型肺的分子机制。方法:40只SPF级SD大鼠建立急性肺损伤模型,将大鼠分为对照组(n=10)、模型组(n=10),阴性对照(Negative control,NC)组(n=10)和Rspondin3组(n=10),模型组、NC组、Rspondin3组建立LPS诱导的大鼠急性肺损伤模型,NC组和Rspondin3建立模型后分别通过尾静脉注射(Negative control,NC)质粒和Rspondin3过表达质粒,对照组和模型组给与等量生理盐水灌,2周后,HE染色检测各组大鼠病肺组织理病变水平,通过Elisa实验检测各组大鼠大鼠肺组织白细胞介素-1(Interleukin-1,IL-1),白细胞介素-2(Interleukin-2,IL-2),白细胞介素-6(Interleukin-6,IL-6)水平的变化,Western blot检测大鼠肺组织wnt-3a及β-环联蛋白(β-catenin)蛋白表达水平,透射电镜技术检测各组大鼠肺组织超微结构变化。结果:与对照组相比,模型组和NC组组织可见明显肺组织损伤,Rspondin3组大鼠肺组织病理损伤程度相对降低,Elisa结果表明,与对照组相比,模型组、NC组和Rspondin3组大鼠肺组织炎症因子IL-1、IL-2和IL-6表达水平升高,与模型组和NC组相比,Rspondin3组大鼠肺组织炎症因子IL-1、IL-2和IL-6表达水平降低,差异具有显著的统计学意义(P<0.01)。Western blot结果表明,与对照组相比,模型组、NC组大鼠Wnt-3a和β-catenin蛋白表达水平降低,与模型组和NC组相比,Rspondin3组大鼠肺组织中Wnt-3a和β-catenin蛋白表达升高降低,差异具有显著的统计学意义(P<0.01)。透射电镜技术表明,与对照组相比,模型组和NC组组织肺组织结构松散,细胞核崩解,细胞结构不完整,细胞空泡化,而Rspondin3组大鼠肺组超微结构表明细胞结构损伤降低,细胞核崩解缓解,细胞结构相对完整,结论:过表达Rspondin3对大鼠急性肺损伤肺组织具有保护作用,其机制可能与激活Wnt-3a/β-catenin信号通路的激活有关。Objective:To investigate the expression of vascular secretory factor Rspondin3 through Wnt/β-catenin signaling pathway in the molecular mechanism of acute lung injury rat model.Methods:The acute lung injury model of SD rats was established.The rats were divided into control group(n=10),model group(n=10),NC group(n=10)and Rspondin3 high group(n=10).The LPS induced acute lung injury model of rats was established in the model group,NC group and Rspondin3 group.After the NC group and Rspondin3 were established,NC plasmid and Rspondin3 over-expression plasmid were injected through tail vein respectively.The control group and model group were given the same amount of normal saline.After 2 weeks,HE staining was used to detect the level of pathological changes in the lung tissues of rats in each group.ELISA was used to detect the changes of interleukin-1(interleukin-1,IL-1),interleukin-2(interleukin-4,IL-2)and interleukin-6(interleukin-8,IL-6)levels in the lung tissues of rats in each group.Western blot was used to detect Wnt-3a andβ-catenin protein expression level.The ultrastructural changes of lung tissue in each group were detected by transmission electron microscopy.Results:Compared with the control group,the model group and NC group showed obvious lung tissue injury,and the degree of lung tissue pathological injury in Rspondin3 group was relatively reduced.ELISA results showed that compared with the control group,the expression levels of inflammatory factors IL-1,IL-2 and IL-6 in the lung tissue of model group,NC group and Rspondin3 group were increased,and compared with the model group and NC group,the expression levels of inflammatory factors IL-1,IL-2 and IL-6 in the lung tissue of Rspondin3 group were decreased.The difference was statistically significant(P<0.01).Western blot results showed that Wnt-3a andβ-catenin protein expression levels were reduced in the model group and NC group compared to the control group,and Wnt-3a andβ-catenin protein expression was increased and reduced in the lung tissue of the

关 键 词：急性肺损伤 Rspondin3 炎症因子 Wnt-3a信号通路 超微结构 

分 类 号：R563.8[医药卫生—呼吸系统]