三七总皂苷抑制人类风湿关节炎滑膜成纤维细胞系增殖和促凋亡  

Panax Notoginseng Saponins Inhibit the Proliferation and Promote Apoptosis of Human Rheumatoid Arthritis Synovial Fibroblast Cell Line

作　　者：贠丹丹 耿男 李菊萍[2] 刘璞 张婷[1] 刘丹[1] YUN Dandan;GENG Nan(The First Affiliated Hospital of Xi'an Medical University,Shaanxi Xi'an 710077,China)

机构地区：[1]西安医学院第一附属医院风湿免疫科,陕西西安710077 [2]西北政法大学公安学院,陕西西安710061

出　　处：《河北医学》2023年第1期53-58,共6页Hebei Medicine

基　　金：陕西省自然科学基础研究计划资助项目,(编号:2021JZ-59);西安市科技计划项目,[编号:20YXYJ0003(7)];西安市卫生健康委员会项目,(编号:2020ms06)。

摘　　要：目的:探讨三七总皂苷对人类风湿关节炎滑膜成纤维细胞增殖、凋亡、迁移和侵袭的的影响及可能机制。方法:MH7A细胞用不同剂量(0.5、1.0、2.0mg/mL)三七总皂苷干预细胞24h后,CCK-8、流式细胞术、划痕实验和Transwell分别检测细胞增殖、凋亡、迁移和侵袭,蛋白质印迹法检测蛋白(Ki-67、PCNA、E-cadherin和N-cadherin)的表达,qRT-PCR法检测miR-335-5p表达。转染miR-335-5p模拟物、转染miR-335-5p抑制剂至MH7A细胞后再用2.0mg/mL三七总皂苷干预24h,然后采用上述相同方法检测细胞增殖、迁移和侵袭。结果:与对照组比较,三七总皂苷降低MH7A细胞A值、划痕愈合率、侵袭数及Ki-67、PCNA和N-cadherin蛋白表达量(P<0.05),而提高细胞凋亡率、E-cadherin蛋白表达量(P<0.05),同时促进细胞中miR-335-5p表达(P<0.05)。上调miR-335-5p后,MH7A细胞A值、划痕愈合率、侵袭数及Ki-67、PCNA和N-cadherin蛋白表达量均降低(P<0.05),细胞凋亡率、E-cadherin蛋白表达量升高(P<0.05)。下调miR-335-5p逆转了三七总皂苷对MH7A细胞增殖、凋亡、迁移和侵袭的影响。结论:三七总皂苷可能通过上调miR-335-5p抑制类风湿关节炎滑膜成纤维细胞MH7A增殖、迁移和侵袭,并促进细胞凋亡。Objective:To investigate the effect and possible mechanism of Panax notoginseng saponins on the proliferation,apoptosis,migration and invasion of human rheumatoid arthritis synovial fibroblasts.Methods:MH7A cells were intervened with different doses(0.5,1.0,2.0 mg/mL)of Panax notoginseng saponins for 24h,and then cell proliferation,apoptosis,migration and invasion were detected by CCK-8,flow cytometry,scratch assay and Transwell,respectively.The expression of proteins(Ki-67,PCNA,E-cadherin and N-cadherin)were detected by Western blotting,and the expression of miR-335-5p was detected by qRT-PCR.The miR-335-5p mimic was transfected into MH7A cells,or the miR-335-5p inhibitor was transfected into MH7A cells and then intervened with 2.0mg/mL Panax notoginseng saponins for 24h,and then the same methods as above were used to detect cell proliferation,migration and invasion.Results:Compared with the control group,different doses of Panax notoginseng saponins decreased the A value,scratch healing rate,invasion number and the expression of Ki-67,PCNA and N-cadherin protein in MH7A cells(P<0.05),but increased the apoptosis rate and E-cadherin protein expression(P<0.05),and promoted the expression of miR-335-5p in cells(P<0.05).After up-regulating miR-335-5p,the A value,wound healing rate,invasion number and the expression of Ki-67,PCNA and N-cadherin protein in MH7A cells were all decreased(P<0.05),but the apoptosis rate and E-cadherin protein expression was decreased(P<0.05).Down-regulation of miR-335-5p reversed the effects of Panax notoginseng saponins on the proliferation,apoptosis,migration and invasion of MH7A cells.Conclusion:Panax notoginseng saponins may inhibit the proliferation,migration and invasion of rheumatoid arthritis synovial fibroblasts MH7A and promote cell apoptosis by up-regulating miR-335-5p.

关 键 词：三七总皂苷 类风湿关节炎 滑膜成纤维细胞 增殖 凋亡 

分 类 号：R285[医药卫生—中药学]