磷胁迫下内生真菌对米老排生长和磷素吸收的影响  

Effects of Endophytic Fungi on Seedling Growth and Phosphorus Uptake of Mytilaria laosensis Under Phosphorus Stress

作　　者：沈雯 邹秉章 林能庆 代甜甜 林晗[1,2] 洪永辉 林景泉 谢安强 SHEN Wen;ZOU Bingzhang;LIN Nengqing;DAI Tiantian;LIN Han;HONG Yonghui;LIN Jingquan;XIE Anqiang(College of Forestry,Fujian Agriculture and Forestry University,Fuzhou 350002,China;Key Laboratory for Forest Ecosystem Processes and Management in Fujian Province,Fuzhou 350002,China;Shanghang Baisha Forestry Farm,Longyan 364200,China;Longyan City Forestry Seedling Station,Longyan 364200,China)

机构地区：[1]福建农林大学林学院,福建福州350002 [2]福建省高校森林生态系统经营与过程重点实验室,福建福州350002 [3]福建省上杭白砂国有林场,福建龙岩364200 [4]龙岩市林业种苗站,福建龙岩364200

出　　处：《广东农业科学》2022年第12期55-63,共9页Guangdong Agricultural Sciences

基　　金：龙岩市科技计划项目(2019LYF9001);福建农林大学横向科研项目(KH200200A);福建省林业局重点项目(KLb21009B)。

摘　　要：【目的】探究内生真菌对低磷胁迫下米老排(Mytilaria laosensis)幼苗磷元素吸收的影响,筛选出能够促进米老排幼苗生长和磷元素吸收的内生真菌。【方法】以米老排幼苗为研究对象,施浇菌液接种10种供试菌株(M1、M2、M3、M4、M5、M6、M7、M8、M9、M10),15 d后进行磷胁迫试验,胁迫60 d后测定米老排幼苗的苗高、地径、地上部和根系的干质量及磷元素含量、叶片和根系酸性磷酸酶活性以及根际土壤酸性磷酸酶。【结果】随着磷胁迫的增加,菌株M9、M10能够增加米老排幼苗苗高、地径以及根冠比,促进幼苗生长;在正常条件和重度磷胁迫下菌株M6能够有效提高米老排幼苗地上部磷含量,分别为36.9%、41.1%,菌株M9的效果随着磷胁迫程度加深更为突出,在重度磷胁迫下菌株M4能够显著增加米老排幼苗根系磷含量为57.7%;菌株M6和M9均能显著提高米老排幼苗叶片和土壤酸性磷酸酶活性,除轻度磷胁迫外,菌株处理后的米老排幼苗根系酸性磷酸酶活性较CK均有显著提升。【结论】土壤磷胁迫下,接种内生真菌对米老排幼苗生长和磷元素吸收具有显著影响,在不同磷胁迫环境下,菌株M6、M9在一定程度上能够有效提高米老排幼苗磷含量以及酸性磷酸酶、超氧化物歧化酶活性,促进幼苗生长。【Objective】The study was conducted to explore the effects of endophytic fungi on phosphorus uptake in Mytilaria Laosensis seedlings under low phosphorus stress, and to select the endophytic fungi that could promote the growth and phosphorus uptake in M. Laosensis seedlings. 【Method】Taking M. Laosensis seedlings as the research object,10 strains(M1, M2, M3, M4, M5, M6, M7, M8, M9, M10) were inoculated by the method of irrigating bacterial solution. Fifteen days later, low phosphorus stress test(18, 12, 6,0 mg/kg) was conducted, after sixty days of low phosphorus stress, the height and ground diameter, the root dry weight and the phosphorus content of the shoots and roots of plants, the acid phosphatase activity in leaves and roots and rhizosphere soil were measured to explore the growth and phosphorus uptake of M. Laosensis.【Result】With the increase of stress degree, strain M9 and M10 could increase the height, ground diameter and root-shoot ratio of M. Laosensis and promote its growth. Under normal conditions and severe stress, strain M6 could effectively increase the phosphorus content in shoot of M. Laosensis by 36. 9% and 41. 1%, respectively. With the increase of stress degree, strain M9 had a more prominent effect and strain M4 could significantly increase the phosphorus content in the root of M. Laosensis,which increased by 57.7% under severe stress. Under severe stress, both strains M6 and M9 could significantly increase the acid phosphatase activity in leaves of M. Laosensis and soil;except for mild stress, the acid phosphatase activity of the root system of M. Laosensis treated with the strain was significantly higher than that of CK.【Conclusion】Under soil phosphorus stress,inoculation of endophytic fungi has a significant effect on the growth and phosphorus uptake of M. Laosensis. Under different stress environments, strains M6 and M9 can increase phosphorus content, acid phosphatase activity and superoxide dismutase activity in M. Laosensis to a certain extent and promote seedling growt

关 键 词：内生真菌 米老排幼苗 土壤磷胁迫 磷元素吸收 酸性磷酸酶活性 

分 类 号：S792.39[农业科学—林木遗传育种]