二球悬铃木COL5同源基因及其表达模式分析  

Analysis of COL5 homologous genes and the expression pattern in Platanus acerifolia

作　　者：王洋 谢宇 孙海莲 石磊 邱晓 刘亚红 常乐 高凤云 WANG Yang;XIE Yu;SUN Hailian;SHI Lei;QIU Xiao;LIU Yahong;CHANG Le;GAO Fengyun(Center for Comprehensive Test and Demonstration,Inner Mongolia Academy of Agricultural and Animal Husbandry Sciences,Hohhot 010031,China;Huazhong Agricultural University,Wuhan 430070,China;Institute of Special Crops,Inner Mongolia Academy of Agricultural and Animal Husbandry Sciences,Hohhot 010031,China)

机构地区：[1]内蒙古自治区农牧业科学院综合试验示范中心,内蒙古呼和浩特010031 [2]华中农业大学,湖北武汉430070 [3]内蒙古自治区农牧业科学院特色作物研究所,内蒙古呼和浩特010031

出　　处：《北方农业学报》2022年第5期10-18,共9页Journal of Northern Agriculture

基　　金：内蒙古自治区自然科学基金项目(2020MS03095)。

摘　　要：【目的】探究二球悬铃木COL5同源基因在表达水平上与休眠调控的相关性。【方法】选定CO-Like基因家族中的COL5作为研究对象,以二球悬铃木成年植株为材料,设计基因特异引物,对COL5同源基因进行全长扩增;通过一年生二球悬铃木幼苗群体的休眠环境因子调控试验,分析该基因在单一短日照(24℃,8 h/16 h)、单一低温(8℃,12 h/12 h)和低温短日照(8℃,8 h/16 h)条件下的响应表达模式。【结果】PaCOL5-1全长为1444 bp,包括1134 bp CDS区,推测编码377个氨基酸;PaCOL5-2全长为1371 bp,包括1131 bp CDS区,推测编码376个氨基酸;PaCOL5-3全长为1186 bp,包括1125 bp CDS区,推测编码374个氨基酸。单一低温会抑制PaCOL5-1的表达,PaCOL5-1和PaCOL5-3均对低温短日照条件有所响应,在处理7 d后会出现1个峰值;PaCOL5-2对低温短日照条件的响应不明显。【结论】PaCOL5-1、PaCOL5-2和PaCOL5-3基因对单一环境因子的响应表达程度不同,PaCOL5-1和PaCOL5-3基因可能与休眠环境因子的响应有关。【Objective】To investigate the relationship between Platanus acerifolia expression level of COL5 homologous genes and dormancy regulation.【Methods】COL5 in the CO-Like gene family was selected as the research object.Gene-specific primers were used to amplify the full length of COL5 homologous genes in Platanus acerifolia adult plants.Platanus acerifolia annual seedling regulation experiment of dormancy environment was used to analyze the expression pattern of the genes under the conditions of short day light alone(24℃,8 h/16 h),low temperature alone(8℃,12 h/12 h),or low temperature and short day light(8℃,8 h/16 h).【Results】The full length of PaCOL5-1 was 1444 bp,including 1134 bp CDS region,which was presumed to encode 377 amino acids.The full length of PaCOL5-2 was 1371 bp,including 1131 bp CDS region,which was presumed to encode 376 amino acids.The full length of PaCOL5-3 was 1186 bp,including 1125 bp CDS region,which was presumed to encode 374 amino acids.Low temperature alone inhibited the expression of PaCOL5-1.Both PaCOL5-1 and PaCOL5-3 responded to low temperature and short day light conditions,and had a peak after 7 d treatment.The response of PaCOL5-2 to low temperature and short day light conditions was not obvious.【Conclusion】PaCOL5-1,PaCOL5-2,and PaCOL5-3 had different gene expression levels in response to a single environmental stimulus.PaCOL5-1 and PaCOL5-3 genes may be involved in the response of dormant environmental factors.

关 键 词：二球悬铃木 COL5 休眠调控 表达分析 

分 类 号：S687.1[农业科学—观赏园艺]