合肥地区HPA、HLA高分辨基因型血小板供者库的建立及库容分析  

Establishment and capacity analysis of Hefei platelet donor bank with HPA and HLA high-resolution results

作　　者：赵娜娜 王超[1] 庞霄[1] 赵阳[1] 於娟[1] 吕蓉[1] ZHAO Nana;WANG Chao;PANG Xiao;ZHAO Yang;YU Juan;LV Rong(Anhui Blood Center,Hefei Central Blood Station,Hefei 230031,China)

机构地区：[1]安徽省血液中心合肥市中心血站,安徽合肥230031

出　　处：《中国输血杂志》2022年第11期1109-1112,共4页Chinese Journal of Blood Transfusion

基　　金：合肥市科技局2019年自主创新“借转补”项目(J2019Y05)。

摘　　要：目的 建立合肥地区HPA、HLA高分辨已知基因型血小板供者资料库,并分析合适的库容水平,为免疫性血小板输注无效患者提供数据基础。方法 采用多重荧光PCR方法对1 157名合肥地区无偿献血者进行HPA-1~6,-10,-15,-21基因分型并计算其频率;采用PCR-SSO方法对合肥地区1 115名无偿献血者进行HLA-A,-B高分辨基因分型,使用最大期望值计算HLA-A,-B高分辨基因频率和单体型频率。结果 根据对合肥地区人群HPA、HLA高分辨基因多态性分析,建立合肥地区血小板供者资料库,在1 157份已知HPA-1~6,-10,-15,-21基因型供者库中,约有91%的患者可以找到HPA基因型全匹配供者;1 115份已知HLA-A,-B高分辨基因型血小板供者库中,找到至少1例相同供者的概率约为60%。结论 建立了适合合肥地区人群HPA、HLA已知基因型血小板供者资料库,经分析合肥地区血小板供者库基本满足免疫性血小板输注无效患者找到适配的血小板需求。Objective To establish a platelet donor database with HPA and HLA high resolution results in Hefei area, and to analyze the appropriate pool capacity, so as to provide support for patients with immune PTR transfusion difficulties. Methods The HPA-1-6,-10,-15 and 21 genotypes of 1157 voluntary blood donors in Hefei were analyzed by multiplex PCR and the frequencies were calculated. HLA-A,-B high resolution gene frequency and haplotype frequency in 1115 voluntary blood donors in Hefei were calculated using maximum expected value method. Results Hefei database of platelet donors was established according to the analysis of HPA and HLA high-resolution gene polymorphisms in Hefei population.In the donor pool with 1 157 cases of known genotypes HPA-1-6,-10,-15,-21, about 91% of patients can find an donor with full-matched HPA genotype. In the donor pool with 1 115 cases of known HLA-A,-B high-resolution genotype, the probability of finding at least one identical donor is about 60%.Conclusion The local-suitable database of platelet donors with known HPA and HLA genotypes in Hefei has been established. After analysis, this database basically meets the needs of patients with immune PTR to find suitable platelets.

关 键 词：血小板供者 HLA HPA 库容分析 

分 类 号：R331.143[医药卫生—人体生理学] R457.11[医药卫生—基础医学]