贮存时间对红细胞非典型趋化因子受体1清除及储存趋化因子的影响  

Effect of storage time on the chemokine storage and chemokine scavenging function of erythrocyte atypical chemokine receptor 1

作　　者：周世航[1] 王霓[1] 刘铭[2] 梁晓华[1] ZHOU Shihang;WANG Ni;LIU Ming;LIANG Xiaohua(Dalian Blood Center,Dalian 116001,China;Department of Cell Biology,Dalian Medical University)

机构地区：[1]大连市血液中心,辽宁大连116001 [2]大连医科大学细胞生物学教研室

出　　处：《中国输血杂志》2022年第11期1113-1116,共4页Chinese Journal of Blood Transfusion

基　　金：辽宁省自然基金指导计划(2019-ZD-0883)。

摘　　要：目的 探讨贮存时间对红细胞非典型趋化因子受体1(atypical chemokine receptor 1,ACKR1)的趋化因子清除功能及储存的影响。方法 6袋去白细胞悬浮红细胞制剂,每袋通过无菌热合机分出2小袋(10 mL/袋),置于血液贮存冰箱储存,分别于贮存d5、d25取出,进行红细胞CCL5、CXCL8以及CCL11含量以及ACKR1趋化因子清除功能测定。同时采集贮存期在d5~25的42份(1 mL/份)去白细胞悬浮红细胞制剂标本,进行红细胞膜上ACKR1表达及趋化因子CCL5、CXCL8以及CCL11含量的检测。结果 贮存期d 25和d 5相比,红细胞裂解液CCL5(pg/mL)(467.7±250.2 vs 586.9±209.5,P>0.05)及CCL11(pg/mL)(122.2±30.3 vs 125.5±32.7,P>0.05)浓度无差异,而CXCL8的浓度降低(pg/mL)(42.4±5.3 vs 24.3±5.9,P<0.05),红细胞ACKR1对趋化因子CCL5(pg/mL)(2 634.0±730.2 vs 453.8±257.4,P<0.05)、CXCL8(pg/mL)(1 117.0±236.6 vs 306.2±28.3,P<0.05)以及CCL11(pg/mL)的清除能力下降(1 278.0±164.5 vs 467.2±50.9,P<0.05)。在红细胞膜表面未检出趋化因子CCL5、CXCL8以及CCL11。红细胞表面ACKR1表达在d 5~25期间,无明显变化(P>0.05)。结论 红细胞内是贮存ACKR1结合趋化因子主要场所,在红细胞贮存期会发生红细胞ACKR1趋化因子清除功能的降低以及红细胞内部储存的某些ACKR1结合趋化因子的降低。Objective To determine the effect of storage time on the chemokine storage and chemokine scavenging function of erythrocyte atypical chemokine receptor 1(ACKR1). Methods Samples from six bags of red blood cells product, split into two gruoups(10 mL each), were stored in a refrigerator and sampled on day 5 and day 25 during storage, respectively, to measure the concentrations of CCL5, CXCL8 and CCL11 and the ACKR1 chemokine scavenging function of erythrocytes. In addition, 42 erythrocyte products(1 mL each), stored for 5 to 25 days, were sampled to measure the expression of ACKR1 and the concentrations of chemokines CCL5, CXCL8 and CCL11 on the erythrocyte membrane. Results Compared with RBCs stored for 5 days, no difference in the concentration of CCL5(467.7±250.2 pg/mL vs 586.9±209.5 pg/mL,P>0.05) and CCL11(122.2±30.3 pg/mL vs 125.5 ±32.7 pg/mL,P>0.05)were noticed in erythrocyte lysates stored for 25 days, but the concentrations of CXCL8 decreased significantly(42.4±5.3 pg/mL vs 24.3± 5.9 pg/mL,P<0.05), and the scavenging ability of erythrocyte ACKR1 to chemokines CCL5(2 634.0±730.2 pg/mL vs 453.8±257.4 pg/mL,P<0.05), CXCL8(1 117.0±236.6 pg/mL vs 306.2±28.3 pg/mL,P<0.05) and CCL11 decreased(1 278.0 ±164.5 pg/mL vs 467.2 ±50.9 pg/mL,P<0.05). Chemokines CCL5, CXCL8 and CCL11 had not been detected on the surface of erythrocyte membrane. There was no significant change in the expression of ACKR1 on the surface of erythrocytes from day 5 to day 25(P>0.05). Conclusion Erythrocytes are the main places for storing ACKR1 binding chemokines. During the storage, the chemokine scavenging of erythrocyte ACKR1 and some intracellular the ACKR1 binding chemokines are reduced.

关 键 词：非典型趋化因子受体1 趋化因子 红细胞 

分 类 号：R457.11[医药卫生—治疗学]