陕西省Kidd、Diego、Duffy血型数据库的建立及临床应用研究  

Establishment and clinical application of Kidd, Diego, Duffy blood group system, Shaanxi Province

作　　者：褚晓月 张泳[1] 毛娟[1] 张良子 左琴琴[1] 张薇薇 吴大洲[1] 王红[1] 徐华[1] CHU Xiaoyue;ZHANG Yong;MAO Juan;ZHANG Liangzi;ZUO Qinqin;ZHANG Weiwei;WU Dazhou;WANG Hong;XU Hua(Shaanxi Blood Center,Xi'an 710061,China)

机构地区：[1]陕西省血液中心,陕西西安710061

出　　处：《中国输血杂志》2022年第11期1124-1127,共4页Chinese Journal of Blood Transfusion

基　　金：2022年陕西省卫生健康科研项目(2022D050);西安市卫生健康委员会科研项目(2020yb47;J201802020)。

摘　　要：目的 在陕西省无偿献血人群中筛查Kidd、Diego、Duffy血型系统稀有表型个体并建立稀有血型数据库与实体库。方法 采用2 mol/L尿素溶血实验筛查Jk(a-b-)表型献血者;采用基因分型方法筛查Di(a+b-)表型献血者;采用抗球蛋白法筛查Fy(a-)和D--表型献血者。结果 在158 484名无偿献血中筛查到Jk(a-b-)表型3例,陕西地区Jk(a-b-)表型分布频率为0.019‰;在4 586名无偿献血中筛查到Di(a+b-)表型2例,陕西地区Di(a+b-)表型分布频率为0.436‰;在1 983名无偿献血中筛查到Fy(a-)表型8例,陕西地区Fy(a-)表型分布频率为4.034‰;在29 430名无偿献血中未筛查到D--表型。结论 通过大样本量的检测建立Kidd、Diego、Duffy稀有血型献血者资料库,得出本地区人群Kidd、Diego、Duffy红细胞血型的分布规律及遗传特征,并在条件成熟时利用红细胞冻存技术建立稀有血型实体库,合理储备血液,为临床患者提供最相合的血液,能够有效提高临床输血安全水平,并为血液预警提供数据支持。Objective To screen individuals with rare blood type of Kidd, Diego, Duffy blood group system among the voluntary blood donor in Shaanxi province and to establish on-line and physical database of rare blood type. Methods Jk(a-b-)phenotype donors were screened by 2 mol/L urea hemolysis test. Blood donors with Di(a+b-) phenotype were screened by genotyping;Fy(a-) and D--phenotype donors were screened by modified antiglobulin assay.Results Three cases of Jk(a-b-) phenotype were detected out of 158 484 voluntary blood donors. The distribution frequency of Jk(a-b-) phenotype was 0.019‰. Di(a+b-) phenotype was detected in 2(0.436‰) cases out of 4 586 voluntary blood donor. Fy(a-) phenotype was detected in 8(4.034‰) cases out of 1 983 voluntary blood donors. D--phenotype was not detected in 29 430 voluntary blood donors. Conclusion The on-line database of Kidd, Diego, Duffy blood group system had been established by large-size screening of blood donor samples, which can conclude the region′s population distribution and genetic characteristics of RBC blood group. And physical database could further be established using the technology of red blood cells cryopreservation when the conditionspermit, so as to provide the most compatible blood for the clinical effectively improve blood transfusion safety, and provide data support for blood early warning.

关 键 词：稀有血型 数据库 红细胞冻存 临床应用研究 

分 类 号：R457.11[医药卫生—治疗学]