HBV DNA反应性献血者血清学与核酸定量检测的结果分析  

Result analysis of serology test and NAT quantitative detection of HBV DNA reactive blood donors

作　　者：魏胜男[1] 彭冬菊[1] 田方[1] WEI Shengnan;PENG Dongju;TIAN Fang(Shiyan Blood Center,Shiyan 442000,China)

机构地区：[1]十堰市中心血站,湖北十堰442000

出　　处：《中国输血杂志》2022年第11期1167-1169,共3页Chinese Journal of Blood Transfusion

基　　金：湖北省卫生健康委联合基金项目(WJ2019H334)。

摘　　要：目的 了解十堰地区无偿献血人群中HBV DNA反应性标本相关标志物的分布情况,为献血者归队提供必要的数据依据。方法 收集2019年8月~2021年6月本站无偿献血者HBV DNA反应性标本共78份,对其进行乙肝血清学标志物、病毒核酸定量以及病毒基因测序等检测。结果 78份HBV DNA反应性标本的献血者中60.3%(47/78)为重复献血者,且以年龄>45岁者居多,占56.4%(44/78)。乙肝血清学标志物检测结果中单抗-HBs阳性占37.2%(29/78),5项全阴性占11.5%(9/78),抗-HBe+抗-HBc和抗-HBs+抗-HBe+抗-HBc阳性占19.2%(15/78);病毒核酸定量62.8%(49/78)的标本检出;7份标本经测序验证含有HBV序列。结论 核酸检测可以有效减少HBsAg的漏检,降低经血传播HBV风险;对HBV DNA阳性献血者的归队还需要慎重对待。Objective To investigate the related viral markers of HBV DNA reactive samples among voluntary blood donors in Shiyan area, so as to provide some reference for donor re-entry. Methods From August 2019 to June 2021, 78 samples with HBV DNA reactivity from voluntary blood donors in our blood station were collected, and they were further detected for HBV viral markers(using chemiluminescence assays), nucleic acid testing(NAT) of virus quantification and sequencing analysis. Results Forty-seven(60.3%)out of 78 HBV DNA reactive samples were from repeated blood donors, and 56.4%(44/78)of them were over 45 years old. Among the five viral markers of hepatitis B, 37.2%(29/78) were positive for anti-HBs alone, 19.2%(15/78) were positive for anti-HBe+anti-HBc and anti-HBs+anti-HBe+anti-HBc, and 11.5%(9/78) were all items negative.A total of 62.8%(49/78) of samples were detected by NAT quantification and seven samples had been successfully sequenced for HBV. Conclusion NAT can effectively minimize the missed detection of HBsAg methodology and reduce the risk of HBV transmission through blood. The re-entry of HBV DNA reactive blood donors should be treated with care.

关 键 词：无偿献血者 HBV 血清学 核酸检测 补充试验 

分 类 号：R457.1[医药卫生—治疗学]