红细胞保存液对特定患者血清凝集反应的影响  

Effect of red blood cell preservation solution on serum agglutination reaction of specific patients

作　　者：郝萧 马立宁 柳华南 夏小叶 刁雪芹 李雪晶 HAO Xiao;MA Lining;LIU Huanan;XIA Xiaoye;DIAO Xueqin;LI Xuejing(Jinan Blood Station,Jinan 250001,China;Jinan Fourth People's Hospital,Jinan 250001,China)

机构地区：[1]济南市血液供保中心,山东济南250001 [2]济南市第四人民医院

出　　处：《中国输血杂志》2022年第11期1174-1176,共3页Chinese Journal of Blood Transfusion

基　　金：济南市临床重点专项(输血医学)。

摘　　要：目的 探讨8例产生试剂红细胞保存剂抗体、正反定型不一致及不规则抗体阳性患者的输血前检查的注意事项,以保障输血安全。方法 根据生理盐水有无洗涤试剂红细胞,可分为2组进行试验,1组为未经处理的反定型细胞、抗体筛选细胞及鉴定细胞,另外1组为生理盐水洗涤的反定型细胞、筛选细胞及鉴定谱细胞,采用微柱凝胶法、盐水介质法、聚凝胺法进行实验来分析红细胞保存液对特定患者血清凝集反应的影响。结果 8名患中1例正定型为AB(+),4例为B (+),3例为A(+),反定型均受到干扰,8名患者血浆与未经洗涤的反定型细胞(盐水试管法)、筛选细胞及谱细胞(盐水试管法与卡式法)均凝集,凝聚胺法不受影响;与经盐水洗涤的反定型细胞(盐水试管法)、筛选细胞及谱细胞(盐水试管法与卡式法)干扰排除。结论 试剂细胞保存液影响8名患者的血型鉴定,不影响患者输血,无不良输血反应。Objective To explore the precautions of pre-transfusion examination in patients with antibodies to erythrocyte protective solution, discrepant ABO blood typing results, and positive unexpected antibodies, so as to ensure the safety of blood transfusion. Methods The screen cells were divided into two groups according to the presence or absence of washing reagent red blood cells in normal saline. One group had untreated forward typing cells, antibody screening cells and identification panel, and the other group had saline-washed reverse typing cells, antibody screening cells and identification panel. The experiments were carried out by microcolumn gel method, saline medium method and polyamine method to analyze the effect of red blood cell preservation solution on serum agglutination reaction of specific patients. Results Among the 8 patients, forward typing was AB(+) in 1 patient, B(+) in 4, and A(+) in 3, and the reverse typing were interfered. The plasma of 8 patients agglutinated with unwashed reverse typing cells(saline tube method), screen cells and identification panels(saline tube method plus cassette method), while not agglutinated with the polybrene method. The interference was eliminated as using washed reverse typing cells(salinetube method), screen cells and identification panels(saline tube method plus cassette method). Conclusion The erythrocyte preservation solution affected patients’ blood group typing, but not affected the outcome of blood transfusion as no adverse reactions occurred.

关 键 词：试剂红细胞保存剂 交叉配血 正反不符 

分 类 号：R446.1[医药卫生—诊断学]