22个烤烟品种苗期对增强UV-B辐射的耐性分析及鉴定指标筛选  

Analysis of the tolerance of 22 flue-cured tobacco varieties to enhanced UV-B radiation at seedling stage and screening of identification indices

作　　者：顾浩 张锦韬 陈颐 李嘉亮 蔡虓 杨志新[1] 王力 何承刚[1] GU Hao;ZHANG Jintao;CHEN Yi;LI Jialiang;CAI Xiao;YANG Zhixin;WANG Li;HE Chenggang(College of Tobacco Science,Yunnan Agricultural University,Kunming 650201,China;China Tobacco Hunan Industrial Co.,Ltd.,Changsha 410007,China;Yunnan Academy of Tobacco Agricultural Sciences,Kunming 650021,China)

机构地区：[1]云南农业大学烟草学院,昆明市盘龙区650201 [2]湖南中烟工业有限责任公司,长沙市雨花区410007 [3]云南省烟草农业科学研究院,昆明市五华区650021

出　　处：《中国烟草学报》2022年第6期94-103,共10页Acta Tabacaria Sinica

基　　金：湖南中烟工业有限责任公司烟草农业示范与推广科技项目(No.202143000934114);云南省烟草公司科技计划重点项目(No.2020530000241025)。

摘　　要：【目的】对22个烤烟品种苗期耐UV-B辐射能力进行综合评价,并筛选耐性鉴定指标。【方法】测定22个烤烟品种苗期在自然UV-B辐射(对照)和4.60 kJ/m~2·d增强UV-B辐射下的7项生长指标和9项生理指标,依据各单项指标对增强UV-B辐射的响应指数进行主成分分析,并通过隶属函数和系统聚类等分析方法,对22个烤烟品种苗期耐UV-B辐射能力进行综合评价,再结合逐步回归分析筛选烤烟苗期耐UV-B辐射鉴定指标。【结果】将16个单项指标进行主成分分析,得到5个相互独立的综合指标,通过隶属函数法计算得出各品种对增强UV-B辐射耐性评价值(D),利用聚类分析,筛选出7个对UV-B辐射耐性较强、4个对UV-B辐射耐性中等、7个对UV-B辐射耐性弱、4个对UV-B辐射较敏感的烤烟品种,采用逐步回归分析建立烤烟苗期对UV-B辐射耐性预测方程:D’=0.510+0.233SFW+0.135SOD+0.114UV-AS+0.113LDM-0.068W-SS+0.073RDM(R^(2)=0.974,P<0.05),其平均预测精度为95.24%。【结论】NC102、云烟87、云烟85、NC82、中烟201、NC297和中烟100对UV-B辐射耐性较强,中烟103、云烟201、云烟97和云烟203对UV-B辐射较敏感;筛选出幼苗鲜质量(SFW)、超氧化物歧化酶(SOD)活性、紫外吸收物质(UV-AS)含量、叶干质量(LDM)、水溶性糖(W-SS)含量和根干质量(RDM)作为烤烟苗期耐UV-B辐射鉴定指标。[Purpose]This study aims to evaluate the tolerance of 22 flue-cured tobacco varieties to UV-B radiation at seedling stage and screen the identification indices.[Methods]Seven growth indices and nine physiological indices of 22 flue-cured tobacco cultivars were measured under natural UV-B radiation(control)and enhanced UV-B radiation(4.60 kJ/m2·d)at seedling stage.Principal component analysis was carried out according to the response indices of each single index to enhanced UV-B radiation,the UV-B radiation tolerance of 22 flue-cured tobacco varieties at seedling stage was comprehensively evaluated by means of membership letter and systematic cluster analysis,and the identification indices of UV-B radiation tolerance of flue-cured tobacco at seedling stage were selected by stepwise regression analysis.[Results]Through principal component analysis of 16 single indices,five independent comprehensive indices were obtained.The evaluation value(D)of enhanced UV-B radiation tolerance of each variety was calculated by membership function method.Using cluster analysis,seven flue-cured tobacco varieties with strong UV-B radiation tolerance,four with medium UV-B radiation tolerance,seven with weak UV-B radiation tolerance and four with relatively weak UV-B radiation tolerance were selected,and the prediction equation of flue-cured tobacco’s tolerance to UV-B radiation at seedling was established by stepwise regression analysis:D’=0.510+0.233SFW+0.135SOD+0.114UV-AS+0.113LDM-0.068W-SS+0.073RDM(R^(2)=0.974,P<0.05).The average predicted accuracy was 95.24%.[Conclusion]NC102,Yunyan 87,Yunyan 85,NC82,Zhongyan 201,NC297 and Zhongyan 100 high higher tolerance to UV-B radiation,while Zhongyan 103,Yunyan 201,Yunyan 97 and Yunyan 203 are more sensitive to UV-B radiation.Seedling fresh weight(SFW),sup eroxide dismutase(SOD)activity,UV absorbing substance(UV-AS),leaf dry mass(LDM),water-soluble sugar(W-SS)content and root dry mass(RDM)were selected as the evaluation indices of UV-B radiation resistance of flue-cured tobacco at seed

关 键 词：UV-B辐射 烤烟品种 苗期 综合评价 

分 类 号：S572[农业科学—烟草工业]