Semliki森林病毒衍生的DNA疫苗与常规DNA疫苗的比较  

A Comparative Study on SFV-based DNA Vaccine and the Conventional DNA Vaccine

作　　者：邓瑶[1] 孟昕[1] 许洪林[1] 王世峰[1] 陆柔剑[1] 王文玲[1] 谷淑燕[1] 阮力[1] 

机构地区：[1]中国疾病预防控制中心病毒病预防控制所,北京100052

出　　处：《病毒学报》2002年第4期325-331,共7页Chinese Journal of Virology

基　　金：国家自然科学基金(No.39980026)

摘　　要：比较Semliki森林病毒(SFV)衍生的复制型DNA疫苗载体(复制型载体)和常规非复制型DNA疫苗载体(非复制型载体)的导入效率、表达效率、诱导凋亡率和免疫效果,从而评价其作为DNA疫苗载体的应用前景。使用等量SFV载体和常规DNA载体同等效率转染细胞后,复制型载体表达强度比非复制型载体高约3倍,其诱导凋亡的能力是非复制型载体的11倍;以不同剂量的SFV载体和常规DNA载体分别转染BHK21细胞,复制型载体各剂量组载体的表达量均高于非复制型载体。复制型载体在1μg组出现峰值,而非复制型载体则出现在4μg组。体内免疫的结果表明,SFV载体pSCA-SS1免疫的各组小鼠中,低剂量1μg组小鼠的总抗体滴度高于10μg和100μg剂量组;1μgpSCA-SS1免疫的小鼠产生的总抗体滴度与CTL水平,分别与pcDNA3-SS1免疫的小鼠中10μg和100μg组相当。但10μg、100μg组pSCA-SS1免疫小鼠的总抗体及CTL水平,都低于pcDNA3-SS1免疫的小鼠的10μg、100μg组。结果提示:SFV衍生的复制型DNA疫苗载体,在低剂量组时即可诱生与常规DNA疫苗载体高剂量组相近的免疫效果。The purpose of this paper is to explore the applicative prospect of Semliki Forest virus(SFV)based replicative DNA vaccine by comparing its efficiency of transfection and expression,the rate of apoptosis and immune response with conventional nonreplicative DNA vaccineThe expression level of GFP and the rate of apoptosis of SFVbased DNA vector pSCAGFP were 3 times and 11 times as high as that of conventional DNA vector pGLGFP when transfecting cell with equal quantity,respectivelyThe expression level of the former was higher,with a peak on 1μg,than the latter with the peak on 4μg,at the same dosage in every group when using different transfection dosages.The total SS1 antibody titre of pSCASS1 induced at low dosage(1μg)was higher than the other two high dose groups(10μg & 100μg)The total antibody and CTL responses induced in mouse immuned with 1μg pSCA was equal to that immuned with 10μg and 100μg pcDNA3,respectivelyThe total antibody and CTL responses induced in mouse immuned with 10μg and 100μg of pSCASS1 was lower than that immuned with 10μg and 100μg of pcDNA3SS1The results suggest that SFVbased replicative DNA vaccine used in lower dosage is more efficient and safe than the conventional DNA vaccine

关 键 词：Semliki森林病毒 DNA疫苗 比较 复制子 凋亡 载体 

分 类 号：R373.3[医药卫生—病原生物学]