长春市儿童医院1998～2001年婴幼儿杯状病毒腹泻流行病学研究  

The Epidemiological Study of Human Calicivirus among Children with Acute Diarrhea in Changchun Children's Hospital in 1998-2001

作　　者：谢华萍[1] 方肇寅[1] 王光[2] 孙利炜[2] 吕红霞[1] Zhong WM Tai JH 林思恩[1] 童志礼[1] 章青[1] Glass RI Jiang X 

机构地区：[1]中国疾病预防控制中心病毒病预防和控制所,北京100052 [2]长春市儿童医院,长春130061 [3]Children's Hospital Medical Center [4]Centers for Disease Control an dPrevention

出　　处：《病毒学报》2002年第4期332-336,共5页Chinese Journal of Virology

基　　金：美国NIH课题(RO3TWO1192)资助

摘　　要：人类杯状病毒(humancalicivirus,HuCV)是引起儿童和成人非菌性胃肠炎的主要病原之一。为了掌握HuCV在我国的流行情况,1998年7月至2001年6月,从长春市儿童医院2343例5岁以下腹泻患儿中共收集粪便标本1264份,其中1056份来自2135例住院患儿。对轮状病毒检测为阴性的588份标本,经多价酶免疫试验(EIA)和两组引物反转录-聚合酶链反应(RT-PCR)检测HuCV,202份为阳性,其中住院患儿标本178份,HuCV检出率为16 9%。HuCV腹泻以2岁以下儿童为主(占96%),流行高峰季节为11月至次年3月。选择17株HuCV进行分子鉴定,15株属GⅡ 4群,1株属GⅡ 3群,另1株属GⅠ 2群,表明GⅡ 4群HuCV是我国流行的优势株。根据HuCV住院患儿的监测资料初步估计,HuCV腹泻住院率约为0 5‰～2 4‰。讨论了长春地区HuCV的流行趋势和疾病负担。以上结果为我国HuCV腹泻的预防和控制提供了科学依据。Human calicivirus(HuCV)is one of the important pathogens of viral diarrhea among children and adultsWe conducted hospitalbased HuCV surveillance among children under 5 years of ageBetween July 1998 to June 2001,1,264 fecal specimens were collected from changchun children's hospital of which 1,056 stools were from 2,135 hospitalized children202 HuCVs were identified in 588 rotavirusnegative specimens by using both polyvalent EIA and RTPCR with two sets of primer pairsAmong them,178 HuCVs were from inpatients with a positive rate of 17%(178/1056),24 HuCVs from outpatients with positive rate of 115%(24/208).HuCV detection was the highest among infants aged 12-17 months(30%),followed by 9-11months(22%)and 6-8 months(16%)A consistent epidemic occurred during the winter(November through the next March).Of 17 HuCV strains that were cloned and sequenced,15 belonged to Norwalklike virus(NLV)genotype GⅡ-4,1 was NLV genotype GⅡ-3,and 1 was NLV genotype GⅠ-2,which suggested GⅡ-4 HuCVs were the prevalent strains circulating in ChangchunThe rough estimated rate of HuCVattributed hospitalization was 05/1000-24/1000The present findings provide epidemiological background for prevention and control of HuCV diarrhea in China

关 键 词：长春市 儿童医院 1998-2001年 婴幼儿 杯状病毒 腹泻 流行病学研究 

分 类 号：R512.5[医药卫生—内科学] R725.[医药卫生—临床医学]