脂联素对骨质疏松大鼠椎体磷酸钙骨水泥强化后骨质量及生物力学的影响及机制  

Effect and Mechanism of Adiponectin on Bone Quality and Biomechanics of Vertebral Body Strengthened with Calcium Phosphate Cement in Osteoporosis Rats

作　　者：张鑫[1] 马朋朋[1] 刘肃[1] 李伟[1] 张麦粒 赵立春 张春玲[1] Zhang Xin;Ma Pengpeng;Liu Su;Li Wei;Zhang Maili;Zhao Lichun;Zhang Chunling(Department of Orthopeadic Surgery,The First Affiliated Hospital of Hebei North University,Zhangjiakou 075000,China)

机构地区：[1]河北北方学院附属第一医院骨外科,河北张家口075000

出　　处：《实用骨科杂志》2022年第12期1084-1088,共5页Journal of Practical Orthopaedics

基　　金：河北省卫生健康委员会科研基金项目(20220612)。

摘　　要：目的研究脂联素(adiponectin,APN)对骨质疏松大鼠椎体磷酸钙骨水泥(calcium phosphate cement,CPC)强化后骨质量及生物力学的影响及机制。方法采用双侧卵巢切除术制备骨质疏松模型大鼠,共30只,随机分为对照组、1 mg/kg APN组、2 mg/kg APN组,均行第4腰椎CPC强化,7 d后分别给予生理盐水、1 mg/kg APN、2 mg/kg APN局部注射,连续4周。检测第4腰椎骨密度、骨小梁体积分数(bone volume to total volume,BV/TV)、平均骨小梁厚度(trabecular thickness,Tb.Th)、平均骨小梁数目(trabecular number,Tb.N)、最大符合及刚度,碱性磷酸酶(alkaline phosphatase,ALP)、骨钙素(osteocalcin,OCN)、骨桥蛋白(osteopontin,OPN)、磷酸化p38MAPK(phosphorylated p38MPAK,p-p38 MAPK)、磷酸化JNK(phosphorylated JNK,p-JNK)、磷酸化ERK1/2(phosphorylated ERK1/2,p-ERK1/2)的相对表达水平。结果1 mg/kg APN组、2 mg/kg APN组CPC强化椎体的骨密度、BV/TV、Tb.N、Tb.Th、最大负荷、刚度,ALP、OCN、OPN的mRNA相对表达量及蛋白相对表达量,p-p38MPAK、p-JNK、p-ERK1/2的蛋白相对表达量均高于对照组。结论APN增强骨质疏松大鼠椎体CPC强化后的骨质量和生物力学强度,激活p38MPAK、JNK、ERK1/2通路是相关的分子机制。Objective To study the effect and mechanism of adiponectin(APN)on bone quality and biomechanics of vertebral body strengthened with calcium phosphate cement(CPC)in osteoporosis rats.Methods Thirty osteoporosis model rats were prepared by bilateral oophorectomy.They were randomly divided into control group,1 mg/kg APN group and 2 mg/kg APN group.All rats were strengthened with CPC of the fourth lumbar vertebra.7 days later,they were given local injection of normal saline,1 mg/kg APN and 2mg/kg APN respectively for 4 weeks.Bone mineral density,trabecular volume fraction(BV/TV),average trabecular thickness(Tb.Th),average trabecular number(Tb.N),maximum coincidence,stiffness and relative expression levels of alkaline phosphatase(ALP),osteocalcin(OCN),osteopontin(OPN),phosphorylated p38MAPK(p-p38 MAPK),phosphorylated JNK(p-JNK),phosphorylated ERK1/2(p-ERK1/2)were measured.Results Bone mineral density,BV/TB,Tb.Th,Tb.N,maximum coincidence,stiffness and mRNA relative expression levels of ALP,OCN,OPN and protein relative expression levels of ALP,OCN,OPN,p-p38mpak,p-JNK and p-ERK1/2 were higher than those of the control group.Conclusion APN enhances the bone mass and biomechanical strength of vertebral body strengthened with calcium phosphate cement in osteoporosis rats,and the activation of p38MAPK,JNK,ERK1/2 pathways are related molecular mechanisms.

关 键 词：骨质疏松 椎体压缩性骨折 磷酸钙骨水泥 脂联素 信号通路 

分 类 号：R683[医药卫生—骨科学]