2018年四川省洪雅县奶牛场结核感染情况调查  

Investigation on the infection of tuberculosis in dairy farms in Hongya County of Sichuan Province in 2018

作　　者：张书[1] 阳爱国[2] 侯巍[2] 郭莉 张良[4] 杨秀兰[4] 刘艳 吴玉梅 饶正远[1] 陈闯[1] ZHANG Shu;YAMG Ai-guo;HOU Wei;GUO Li;ZHANG Liang;YANG Xiu-lan;LIU Yan;WU Yu-mei;RAO Zheng-yuan;CHEN Chuang(Sichuan Center for Disease Control and Prevention,Chengdu 610041,Sichuan Province,China;Sichuan Animal Epidemic Disease Prevention and Control Center,Chengdu 610000,Sichuan Province,China;Sichuan Veterinary Drug Supervision Institute,Chengdu 610000,Sichuan Province,China;Hongya Center for Disease Control and Prevention,Meishan 620300,Sichuan Province,China;Hongya Center for Animal Epidemic Disease Prevention and Control,Meishan 620300,Sichuan Province,China)

机构地区：[1]四川省疾病预防控制中心,成都610041 [2]四川省动物疫病预防控制中心,成都阝610000 [3]四川省兽药监察所,成都610000 [4]洪雅县疾病预防控制中心,四川眉山620300 [5]洪雅县动物疫病预防控制中心,四川眉山620300

出　　处：《寄生虫病与感染性疾病》2022年第4期190-194,共5页Parasitoses and Infectious Diseases

摘　　要：目的结核病是一种由结核分枝杆菌复合群引起的人畜共患慢性传染病。本研究通过调查四川省洪雅县奶牛场工作人员及奶牛结核分枝杆菌感染情况,为结核病防控提供建议。方法2018年对洪雅县奶牛场378名工作人员进行结核病检查,对奶牛场所有奶牛(10224头)进行结核菌素皮内变态反应试验,培养疑似结核病患者痰标本、结核菌素试验阳性牛及密接牛的食道-咽部分泌物和阳性牛所在环境样本,并使用PCR扩增检测纯培养物16s rDNA、hsp64、rpoB和16s-23s rRNA间隔区,通过测序比对鉴定纯培养物菌型。结果1名工作人员被诊断为肺结核;99头奶牛为结核菌素试验阳性,阳性率0.97%;培养分离菌株21株,经鉴定均为非结核分枝杆菌,包括18株M.nonchromogenicum,1株M.hiberniae,1株M.arupense和1株M.chitae。结论本研究未从奶牛中检出结核分枝杆菌,经鉴定,结核菌素试验阳性牛感染的菌株为非结核分枝杆菌,使用结核菌素试验检测牛结核感染的方法准确度受限,建议对阳性奶牛采用更准确的检测方法进行复检。Objective Tuberculosis was a zoonotic chronic infectious disease caused by mycobacterium tuberculosis complex(MTBC).The aim of our study was to investigate mycobacterium tuberculosis infection between bovine and humans in dairy farms in Hongya County of Sichuan Province,and to provide advice for tuberculosis prevention and control.Methods All 378 workers from dairy farms in Hongya County were screened for tuberculosis symptoms and examined.Skin tests for purified protein derivatives(PPD)were performed on all cows(n=10224).Sputum samples from suspected TB patients,Esophageal-pharyngeal(OP)secretions from PPD positive cows and their close-coupled cows,and environmental samples were cultured using modified L-J medium,then the pure cultures were tested by PCR to detect 16s rDNA,hsp64,rpoB and 16s-23s r RNA spacer region,sequencing comparison method was used to identify pure culture bacterial type.Results One worker was diagnosed tuberculosis.In total,99 cows showed PPD positive(0.97%),and 21isolates were obtained by culture.BLAST analysis classified these isolates as nontuberculous mycobacteria(NTM)(18 M.nonchromogenicum,1 M.hiberniae,1 M.arupense,and 1 M.chitae).Conclusions Mycobacterium tuberculosis was not detected in dairy cows in this study,PPD test positive cattle were identified as infected with non-tuberculous mycobacterium.The method used to detect TB infection in cows using PPD was not very accurate and a more accurate method is recommended.

关 键 词：人畜共患传染病 结核分枝杆菌 非结核分枝杆菌 奶牛 

分 类 号：R52[医药卫生—内科学]