基于microRNA研究大鼠移居高原对子代心脏的影响  

Study on the Effects of Rat Migration to High Altitude on the Hearts of Their Offspring based on MicroRNA

作　　者：何江 索朗多布杰 格桑曲珍[1] 次旦玉珍 龚嘎蓝孜[1] HE Jiang;Suolang-Duobujie;Gesang-Quzhen;Cidan-Yuzhen;Gongga-Lanzi(Medical College,Tibet University,Lhasa 850000,China;Tibet Autonomous Region Rehabilitation Center for Persons with Disabilities,Lhasa 850000,China)

机构地区：[1]西藏大学医学院,西藏拉萨850000 [2]西藏自治区残疾人康复服务中心,西藏拉萨850000

出　　处：《高原科学研究》2022年第4期66-75,共10页Plateau Science Research

基　　金：西藏大学研究生高水平人才培养计划项目(2020-GSP-S077);西藏大学人才队伍建设项目-高层次人才引进项目(藏教财指[2014]125号)。

摘　　要：目的:探讨子代大鼠高原低氧心肌损伤相关microRNA作用,为高原低氧相关心脏病提供防治新方向。方法:将8只8周龄Wistar大鼠按雌雄比3:1随机合笼分为两组:高原吸氧组和高原缺氧组。高原吸氧组按2 L/min的氧流量每日给与24 h供氧,高原缺氧组饲养在海拔3658 m的高原自然环境中。待母鼠产子鼠后,观察两组子鼠1月龄时血压、心率、心脏质量、心脏脏器指数、血清心肌酶及心脏HE染色结果,并用microRNA全基因表达芯片进行初筛。结果:高原缺氧组子代大鼠心脏质量高于高原吸氧组(P<0.05);高原缺氧组子代大鼠血清心肌酶cTn-Ⅰ(心肌肌钙蛋白-Ⅰ)、CK(肌酸激酶)及CK-MB(肌酸激酶同工酶)高于高原吸氧组(P<0.05);HE染色结果显示高原缺氧组子代大鼠有缺氧病理改变,而高原吸氧组子代大鼠缺氧改变明显减轻。与高原吸氧组比较,高原缺氧组子代大鼠外周血中有27个显著差异表达microRNA(FC>2,P<0.05),其中有16个上调microRNA,11个下调microRNA。发现miR-30a-5p与缺氧性心肌损伤密切相关,预测靶基因GJA1参与心血管系统疾病相关的生物过程。结论:高原低氧可引起移居高原子代大鼠心肌缺氧损伤,miR-30a-5p可能通过调控靶基因GJA1的表达在高原缺氧相关心脏病中发挥重要作用。ObjectiveTo explore the role of microRNA related to high altitude hypoxia myocardial injury in offspring rats,and provide a new direction for prevention and treatment of hypoxic related heart disease at high altitude.MethodsA total of Eight 8-week old Wistar rats were randomly divided into two groups according to females and males ratio of 3:1 high altitude oxygen group and high altitude hypoxia group.high altitude oxygen group was given 24 hours of oxygen daily according to the oxygen flow rate of 2 L/min,and the high altitude hypoxia group was fed in the natural environment of the high altitude at 3658 m above sea level.After the mother rats gave birth,blood pressure、heart rate、heart weight、heart index、serum myocardial enzyme and heart HE staining were observed in the two groups of offspring rats at the age of 1 month,and the microRNA whole gene expression chip was used for preliminary screening.ResultsThe heart weight of the high altitude hypoxia offspring rats group was higher than that of the high altitude oxygen offspring rats group(P<0.05);The serum myocardial enzymes levels of cTn-Ⅰ(Cardiac troponinⅠ)、CK(Creatine kinase)and CK-MB(Creatine kinase isoenzyme)in the high altitude hypoxia offspring rats group were higher than those in the high altitude oxygen offspring rats group(P<0.05);The results of HE staining showed that the offspring of rats in the high altitude hypoxia group had hypoxic pathological changes,while the offspring of rats in the high altitude oxygen group had significantly reduced hypoxic changes.Compared with the high altitude oxygen offspring rats group,there were 27 significantly differentially expressed microRNA in peripheral blood of the high altitude hypoxia offspring rats group(FC>2,P<0.05),16 of which were up-regulated and 11 of which were down-regulated.It was found that miR-30a-5p is closely related to hypoxic myocardial injury,and its target gene GJA1 is predicted to be involved in biological processes related to cardiovascular diseases.ConclusionHigh altitude hypox

关 键 词：高原 低氧 心肌损伤 子代 MICRORNA 

分 类 号：R542.2[医药卫生—心血管疾病]