祁连地区牦牛主要消化道线虫对伊维菌素的抗药性检测  

Major Gastrointestinal Nematodes Resistance to Ivermectin in Qilian Yak

作　　者：雷萌桐[1] 李春花[1] 蔡进忠[1] 索南多杰 李英明 LEI Meng-tong;LI Chun-hua;CAI Jin-zhong;SUO Nanduojie;LI Ying-ming(Academy of Animal and Veterinary Science,Qinghai University,Qinghai Provincial Key Laboratory of Pathogen Diagnosis for Animal Diseases and Green Technical Research for Prevention and Control,Xining 810016,China;Qilian Animal Husbandry and Veterinary Station,Qilian 810400,China;Huzhuzh Animal Husbandry and Veterinary Station,Huzhuzh 810500,China)

机构地区：[1]青海省畜牧兽医科学院,青海省动物疾病病原诊断与绿色防控技术研究重点实验室,青海西宁810016 [2]祁连县畜牧兽医站,青海祁连810400 [3]互助县畜牧兽医站,青海互助810500

出　　处：《青海畜牧兽医杂志》2022年第5期23-26,共4页Chinese Qinghai Journal of Animal and Veterinary Sciences

基　　金：国家重点研发专项(2018YFD0502305);青海省重大科技专项(2016-NK-A7)。

摘　　要：为检测祁连地区牦牛自然感染的主要消化道线虫对伊维菌素的抗药性,采用粪便虫卵减少试验,选取60头自然感染消化道线虫的试验牦牛,随机分为4组,1-3组分别按0.1 mg/kg、0.2 mg/kg、0.3mg/kg.b w剂量口服伊维菌素片剂,4组为阳性对照组。在给药前1 d、给药后7 d和14 d,检查统计给药后虫卵减少率,进行抗药性评价。结果表明:给药后7 d和14 d, 0.1 mg/kg剂量组对奥斯特线虫虫卵减少率分别为96.7%和98.1%,对马歇尔线虫、细颈线虫的虫卵减少率均为100%;0.2 mg/kg剂量组及0.3 mg/kg剂量组对奥斯特线虫、马歇尔线虫、细颈线虫的虫卵减少率均为100%。试验证明祁连地区牦牛主要消化道线虫对伊维菌素尚未产生抗药性。To understand whether the major gastrointestinal nematodes in yak are resistance to ivermectin,60 yaks were randomly divided into 4 groups, ivermectin tablet by 0.1 mg/kg.b w、0.2 mg/kg.b w and 0.3 mg/kg.b w was used respectively, positive control group was set. Fecal samples were collected before administration in the 1 d and after administration in the 7 and 14 d, and the faecal egg count reduction test(FECRT) was used for detecting the resistance to ivermectin anthelmintic. The results showed that 7 and 14 d after administration at dosage of 0.1 mg/kg.b w,the reductive rate of Ostertagia ova was 96.7% and 98.1% respectively, the reductive rate of Marshallagia and Nematodirus ova were both 100 %;7 and 14 d after administration at dosage of 0.2 mg/kg.b w and 0.3 mg/kg.b w, the reductive rate of Ostertagia ova, Marshallagia ova and Nematodirus ova were both 100 %. The test proved that the main gastrointestinal nematodes of Qilian yaks had no resistance to ivermectin.

关 键 词：牦牛 伊维菌素 消化道线虫 粪便虫卵减少试验 抗药性 

分 类 号：S852.7[农业科学—基础兽医学]