柚皮苷通过HIF-1α/VEGF信号促进H型血管抗骨质疏松的研究  

Naringin exerts anti-osteoporosis effect by promoting H-type angiogenesis through HIF-1α/VEGF signaling pathway

作　　者：上官文姬[1] 张跃辉[2] 岳江[3] 汤璐敏[1] 张秋琪 宋佳[2] 邵将[2] SHANGGUAN Wenji;ZHANG Yuehui;YUE Jiang;TANG Lumin;ZHANG Qiuqi;SONG Jia;SHAO Jiang(Traditional Chinese medicine department,Ren Ji Hospital Affiliated to Shanghai Jiao Tong University School of Medicine,Shanghai 200120,China;Spine Center,Xin Hua Hospital Affiliated to Shanghai Jiao Tong University School of Medicine,Shanghai 200082,China;Endocrinology Department,Ren Ji Hospital Affiliated to Shanghai Jiao Tong University School of Medicine,Shanghai 200120,China)

机构地区：[1]上海交通大学医学院附属仁济医院中医科,上海200120 [2]上海交通大学医学院附属新华医院脊柱中心,上海200082 [3]上海交通大学医学院附属仁济医院内分泌科,上海200120

出　　处：《中国骨质疏松杂志》2022年第12期1755-1759,共5页Chinese Journal of Osteoporosis

摘　　要：目的观察柚皮苷对去卵巢骨质疏松大鼠骨组织HIF-1α/VEGF信号通路和H型血管形成的影响,探讨柚皮苷防治骨质疏松症的作用机制。方法建立去卵巢骨质疏松大鼠模型(随机将36只大鼠分为去卵巢组、假手术组和柚皮苷治疗组)。柚皮苷治疗3个月后,检测血清和骨髓上清骨代谢指标和HIF-1α/VEGF信号通路变化,检测骨密度(bone mineral density,BMD)、骨微结构及H型血管变化。结果柚皮苷治疗组大鼠血清中成骨代谢指标[骨特异性碱性磷酸酶(BALP)、骨钙素(BGP)、I型原胶原分子N端前肽(PINP)]升高;而骨吸收标志物血清C端交联肽(CTX)降低;HIF-1α在血清和骨髓上清中表达均升高;VEGF在骨髓上清中表达升高,而血清中无明显改变;BMD和Micro-CT结果显示柚皮苷治疗组大鼠骨密度和骨量增加;同时股骨近端H型血管表达升高。结论柚皮苷可以调节骨代谢,改善骨质疏松大鼠骨密度,发挥抗骨质疏松作用,其作用机制可能是通过调节HIF-1α/VEGF信号通路、促进H型血管形成来实现的。Objective The purpose of this study is to observe the effect of naringin on HIF-1α/VEGF signaling pathway and H-type angiogenesis in ovariectomized rats,and to explore the mechanism of anti-osteoporosis of naringin.Methods Thirty-six rats were randomly divided into three groups:ovariectomized group,sham group,and naringin treatment group.Rat model of osteoporosis was established with bilateral ovariectomy.After 3 months of naringin treatment,the changes of bone metabolism in serum and bone marrow supernatant,HIF-1α/VEGF signaling pathway,bone mineral density(BMD),bone microstructure and H-type vessels were detected.Results Compared to the ovariectomized group,the indicator of osteogenic metabolism including BALP,BGP,PINP were increased and CTX was decreased in serum in naringin treatment group.HIF-1αexpression was increased in both serum and bone marrow.VEGF expression was increased in bone marrow,but not in serum.Micro-CT result showed that BMD and bone mass were increased in naringin treatment group.Corresponding,increased H-vessels in the proximal tibia were observed in naringin treatment group compared with that of ovariectomized group.Conclusion Naringin can regulate bone metabolism,improve BMD and play an anti-osteoporosis role in ovariectomized rats,and this may be achieved by promoting H-type angiogenesis through HIF-1α/VEGF signaling pathway.

关 键 词：柚皮苷 绝经后骨质疏松症 H型血管 HIF-1α/VEGF信号通路 

分 类 号：R285.6[医药卫生—中药学]