光纤耦合微通道反应系统原位监测海水氨氮和亚硝酸盐的研究  

In-situ monitoring of ammonia nitrogen and nitrite in seawater by fiber-coupled micro-channel reaction system

作　　者：姜双城[1,2] 杜虹 郑惠东[1] 汤新华[3,4] 潘文涛 高俊 范丹阳 林琪 吕海霞[4] 林旭聪 Jiang Shuangcheng;Du Hong;Zheng Huidong;Tang Xinhua;Pan Wentao;Gao Jun;Fan Danyang;Lin Qi;LüHaixia;Lin Xucong(Fisheries Research Institute of Fujian,Xiamen 361013,China;College of Science,Shantou University,Shantou 515063,China;Xiamen Standards Scientific Instrument Co.Ltd.,Xiamen 361013,China;Engineering Technology Research Center on Reagent and Instrument for Rapid Detection of Product Quality and Food Safety,Fuzhou 350108,China;Chemistry College,Fuzhou University,Fuzhou 350108,China)

机构地区：[1]福建省水产研究所,福建厦门361013 [2]汕头大学理学院,广东汕头515063 [3]厦门斯坦道科学仪器股份有限公司,福建厦门361013 [4]福建省产品质量与食品安全检测试剂和仪器工程技术研究中心,福建福州350108 [5]福州大学化学学院,福建福州350108

出　　处：《海洋学报》2023年第1期138-146,共9页

基　　金：福建省海洋与渔业结构调整专项(2020HYJG05,2021HYJG09);福建省海洋发展补助资金专项(ZHHY−2019−4,FJHJF−L−2020−8,FJHJF−L−2021−9);福建省高校产学合作项目(2020Y4004);科技部重点专项(SQ2020YFF0426577)。

摘　　要：原位监测海水中氨氮(NH_(3)-N)和亚硝酸盐(NO_(2)^(-))对于水体富营养化评价十分重要。针对现有的氨氮、亚硝酸盐原位监测仪器难以在同一检测模块中分别实现荧光和分光光度分析,仪器高效集成及利用受到限制等问题,本文基于光纤耦合微通道反应系统,通过光波长切割、光纤波导和微通道反应Z型检测池等技术集成和条件优化,提出了在同一光纤偶联微反应系统中实现氨氮荧光分析和亚硝酸盐分光光度分析的原位测定技术。结果表明,该技术实现了荧光和可见光光度法的模式切换并可分别测定NH_(3)-N和定;不同的盐度对氨氮和亚硝酸盐测定影响较小;不同浊度下氨氮测定误差为-6.6%~2.5%,浊度补偿校正后NO_(2)^(-)测定误差小于0.1%。将该仪器应用于养殖海水中开展氨氮和亚硝酸盐的原位分析,仪器原位监测值与实验室方法测定值相符合。本文所提出的技术在同一模块中分别实现了氨氮和亚硝酸盐的荧光和分光光度两种模式的原位监测,为海水营养盐原位监测仪器的集成化设计提供了新的思路。In-situ monitoring of ammonia nitrogen(NH_(3)-N)and nitrite(NO_(2)^(-))in seawater is important for evaluating water eutrophication.To date,it is difficult to achieve fluorescence and spectrophotometric analysis of ammonia nitrogen and nitrite in a single detection module,and the efficient integration and utilization of instruments are limited.Herein,in view of this problem,a fiber-coupled micro-channel reaction system was proposed based on technologies such as optical wavelength cutting,optical fiber waveguide and micro-channel reaction Z-cell detection,and realized the fluorescence detection of ammonia nitrogen and the in-situ determination of nitrite by spectrophotometry in the same detection system.The results showed that the mode switching of fluorescence and visible photometry could be realized and NH_(3)-N and NO_(2)^(-)was accomplished by this technique,respectively.Under the constant temperature condition,the drift value of fluorescence signal in NH_(3)-N measurement was less than-2.0%,and the absorbance intensity of NO_(2)^(-)measurement was stable.Little effect on the determination of ammonia nitrogen and nitrite was provided by different salinity.The acceptable measurement error of ammonia nitrogen under different turbidity ranged in-6.6%to 2.5%,and the measurement error of NO_(2)^(-)after turbidity compensation correction was less than 0.1%.The instrument was applied to the in-situ analysis of ammonia nitrogen and nitrite in aquaculture seawater,and the detection value achieve by this method was consistent with the standard method.This method realized in-situ automatic detection of ammonia nitrogen and nitrite in a same module,and provided a new idea for the better integration of in-situ monitoring instruments for real-time monitoring of NH_(3)-N and NO_(2)^(-)in seawater.

关 键 词：原位监测 光纤耦合 微通道反应系统 氨氮 亚硝酸盐 

分 类 号：O657.3[理学—分析化学]