多指标综合评分联合正交设计优化蒲地灌肠液的提取工艺研究  

Optimization of Water Extraction Technology of Pudi Enema by Multi-index Comprehensive Evaluation Combined with Orthogonal Design

作　　者：王艺红[1] 叶财发 黄燕鹏[1] 陈锦珊[1] 杨静 WANG Yi-hong;YE Cai-fa;HUANG Yan-peng;CHEN Jin-shan;YANG Jing(The 909th Hospital of Joint Logistic Support Force/Dongnan Hospital of Xiamen University,Zhangzhou 363000,China;Office for Drug and Instrument Supervision and Inspection,Zhengzhou Joint Logistic Support Center,Beijing 100071,China)

机构地区：[1]联勤保障部队第九〇九医院/厦门大学附属东南医院,福建漳州1363000 [2]郑州联勤保障中心药品仪器监督检验站,北京2100071

出　　处：《解放军药学学报》2022年第6期497-500,共4页Pharmaceutical Journal of Chinese People's Liberation Army

基　　金：福建中医药大学临床专项校管课题,No.XB2018129。

摘　　要：目的优化蒲地灌肠液的提取工艺。方法以芍药苷、咖啡酸的含量和干浸膏得率为评价指标,采用正交设计试验,考察浸泡时间、料液比和提取总时间对提取结果的影响,确定蒲地灌肠液的最佳提取工艺。结果蒲地灌肠液的最佳工艺条件为浸泡时间20 min,提取2次,每次14倍量溶剂,提取总时间为90 min,该提取工艺下得到的芍药苷含量、咖啡酸含量和干浸膏得率均值分别为663.4μg·ml^(-1)、46.02μg·ml^(-1)和20.34%。结论多指标综合评分法联合正交设计优化蒲地灌肠液提取工艺符合中药多成分多靶点的作用特点,优化的提取工艺稳定可行。Objective To optimize the extraction process of Pudi enema.Methods The contents of paeoniflorin,caffeic acid and the yield of dry extract were used as evaluation indexes.Orthogonal design was used to determine the effects of such factors as the duration of soaking,solid-liquid ratio and total duration of extraction on the extraction results and to select the optimal extraction process of Pudi enema.Results The optimum extraction process of Pudi enema was a soaking time of 20 minutes,two-time extraction,14 times as much dilution each time,and the total duration extraction of 90 minutes.The contents of paeoniflorin and caffeic acid and yield of dry extract were 663.4μg·ml^(-1),46.02μg·ml^(-1)and 20.34%,respectively.Conclusion The optimization of the extraction process with the multi-index comprehensive scoring method is compatible with the multi-component and multi-target characteristics of traditional Chinese medicines,and the optimized extraction process is stable and feasible.

关 键 词：蒲地灌肠液 芍药苷 咖啡酸 多指标综合评分 提取工艺 正交设计 

分 类 号：R943[医药卫生—药剂学]