银翘解毒丸中山银花替代金银花投料掺假研究  

Identify Research on Yin Qiao JieDu Pills in Lonicerae Flos Alternative Lonicerae Japonicae Flos

作　　者：郭娅 王文婷 何世新 GUO Ya;WANG Wen-ting;HE Shi-xin(Chongqing Wanzhou Food and Drug Inspection Institute,Chongqing Key Laboratory of Development and Utilization of Genuine Medicinal Materials in Three Gorges Reservoir Area,Chongqing 404100,China)

机构地区：[1]重庆市万州食品药品检验所,三峡库区道地药材开发利用重庆市重点实验室,重庆404100

出　　处：《海峡药学》2022年第11期44-47,共4页Strait Pharmaceutical Journal

基　　金：2021年度重庆市药品科研项目(渝药监[2021]30号)。

摘　　要：目的 以灰毡毛忍冬皂苷乙为专属性物质,建立检测方法对银翘解毒丸中山银花替代金银花进行鉴别研究。方法 采用HPLC-ELSD法对制剂50%甲醇提取液进行分析,并用UPLC-MS/MS法进行验证。结果 建立的HPLC-ELSD法和UPLC-MS/MS法测定灰毡毛忍冬皂苷乙,线性良好,加样回收率分别为101.8%和97.2%,RSD值分别为1.4%和1.9%。此外,35批银翘解毒丸样品中,有7批检测出灰毡毛忍冬皂苷乙,存在违规投料情况。结论 该方法简便、快速、准确,可用于银翘解毒丸中山银花替代金银花投料鉴别的分析。OBJECTIVE Establishment of testing methods to identify on YinQiao JieDu Pills in Lonicerae Flos alternative Lonicerae Japonicae Flos,which using macranthoidin B as the target substance. METHODS The HPLCELSD was used to analyze the formulation 50% methanol extract,and verified with UPLC-MS/MS. RESULTS The established HPLC-ELSD method and UPLC-MS/MS method were used to determine macranthoidin B with the good linearity,and the recoveries of the sample were 101. 8% and 97. 2%,respectively,and the RSD values were 1. 4% and 1. 9%,respectively. In addition,among 35 batches of Yin Qiao JieDu Pills,7 batches were detected as containing macranthoidin B,indicating that there is a violation of the investment. CONCLUSION This method is simple,fast,accurate,and can be used in the identification research on YinQiao JieDu Pills in Lonicerae Flos alternative Lonicerae Japonicae Flos.

关 键 词：银翘解毒丸 金银花 山银花 灰毡毛忍冬皂苷乙 

分 类 号：R927[医药卫生—药学]