MTT法和LDH法评价纳米银抗菌凝胶的体外细胞毒性  

Evaluation of Cytotoxicity of Nano-Silver Antibacterial Gel in Vitro

作　　者：袁博[1] 张娜[1] 景明[1] YUAN Bo;ZHANG Na;JING Ming(Tianjin Medical Devices Quality Supervision and Inspection Center,Tianjin 300384)

机构地区：[1]天津市医疗器械质量监督检验中心,天津300384

出　　处：《中国医疗器械信息》2022年第23期24-26,31,共4页China Medical Device Information

摘　　要：目的:对纳米银抗菌凝胶进行体外细胞毒性评价。方法:试验选用L929小鼠成纤维细胞,通过MTT试验和LDH试验,对被测物进行研究。结果:MTT试验的LC50为0.047g/mL,LDH试验的LC50为0.048g/mL。结论:纳米银离子会破坏细胞线粒体和细胞膜完整性。Objective:To evaluate the cytotoxicity of nanometer silver antibacterial gel in vitro.Methods:L929 mouse fibroblasts were selected and studied by MTT test and LDH test.Results:LC50of MTT test was 0.047g/mL,and LC50of LDH test was 0.048g/mL.Conclusion:Nano-silver ions can destroy the integrity of mitochondria and cell membranes.

关 键 词：纳米银抗菌凝胶 细胞毒性 MTT试验 LDH试验 

分 类 号：R994.1[医药卫生—毒理学]