中国眼裸藻属(Euglenaria)分类学研究  

A TAXONOMIC STUDY OF EUGLENARIA FROM CHINA

作　　者：姜小蝶 万树冉 庞婉婷[1] 王全喜[1] JIANG Xiao-Die;WAN Shu-Ran;PANG Wan-Ting;WANG Quan-Xi(College of Life Sciences,Shanghai Normal University,Shanghai 200234,China;School of Environmental and Geographical Sciences,Shanghai Normal University,Shanghai 200234,China)

机构地区：[1]上海师范大学生命科学学院,上海200234 [2]上海师范大学环境与地理科学学院,上海200234

出　　处：《水生生物学报》2023年第1期113-120,共8页Acta Hydrobiologica Sinica

摘　　要：为对眼裸藻属Euglenaria Karnkowska, Linton&Kwiatowski进行分类学修订,研究以采自中国的12株3种眼裸藻属物种为材料,基于形态学和分子生物学手段对眼裸藻属进行系统发育研究,鉴定了3种眼裸藻属物种,分别是尾眼裸藻Euglenaria caudata (Hubner) Karnkowska et Linton、项圈眼裸藻Euglenaria anabaena(Mainx) Karnkowska et Linton和棒形眼裸藻Euglenaria clavata (Skuja) Karnkowska et Linton,提供了它们的形态特征及分子数据。基于最大似然法和贝叶斯法构建了系统发育树,以亲缘关系较近的双鞭藻科Eutreptiaceae Hollande物种为外类群,其余的裸藻类分为扁裸藻科Phacaceae Kim, Triemer&Shin和裸藻科Euglenaceae Dujardin物种两大支,眼裸藻属位于裸藻科分支(0.84/-),共包含28株眼裸藻属物种,主要分为3个小分支,每个小支的支持率均高达99%以上,项圈眼裸藻和尾眼裸藻为姐妹分支,亲缘关系较近,然而这两个种的细胞大小和叶绿体数量相差很大,这说明眼裸藻属形态与分子系统发育之间的关系可能并不一致。Euglenaria Karnkowska, Linton & Kwiatowski is a new genus isolated from Euglena Ehrenberg in 2010.Currently, there are 4 species reported in the world. In China’s existing algal taxonomic data, the species of Euglenaria are still classified in Euglena, and their morphological description is vague, making it difficult to distinguish them from some species of Euglena. It is difficult to identify Euglenaria and Euglena, and there is no obvious difference between Euglenaria and some species of Euglena in morphology. With the development of molecular biology, new technical means have been brought for the identification of euglenoid. While further research on the systematic classification of euglenoid has been carried out, the classification status of many euglenoid species has been revised, and Euglenaria is a new genus separated from Euglena at this stage. Our study found that the cells of Euglenaria are generally fusiform without mucocysts, and the Chloroplasts are disc-shaped with pyrenoids and edges irregular with deep grooves. The morphological differences among the species of Euglenaria are mainly reflected in the shape, number of chloroplasts and size of cells. The cells morphology of Eu. clepsydroides are hourglass shaped. The cells of Eu. caudata [length(58.95±12.38) μm, width(28.57±5.82) μm] were the largest. The chloroplast number of Eu. anabaena and Eu. clavata was significantly different. Eu. anabaena contained fewer chloroplasts(only 4±1), while Eu. clavata cells contained10±3 chloroplasts. The morphological differences among species of Euglenaria are very subtle, and it is difficult to correctly identify them. The phylogenetic tree showed that with the closely related Eutreptiaceae as the outgroup, the results indicated that the rest of the euglenoids were divided into two major clades, the Euglenaceae clade and the Phacaceae clade. Euglenaceae(0.96/597) included Euglenaformis, Strombomonas, Trachelomonas, Cryptoglena,Euglena, Monomorphina, Euglenaria and Colacium. Each genus formed a well-suppo

关 键 词：裸藻属 形态特征 系统发育 分类修订 眼裸藻属 

分 类 号：Q949.2[生物学—植物学]