Potyvirus属成员基因组全序列的简并引物PCR和RACE扩增方法  

Determination of Genome Sequence of Potyviruses by Degenerated PCR and RACE Methods

作　　者：陈炯[1] 陈剑平[1] 

机构地区：[1]浙江省农业科学院病毒室,浙江杭州310021

出　　处：《病毒学报》2002年第4期371-374,共4页Chinese Journal of Virology

基　　金：浙江省青年科技人才专项(RC9604);浙江省重点实验室专项(001107557);浙江省十五重点科技项目(011102181)

摘　　要：Based on multi-alignment of complete polyprotein amino acid sequences of genus Potyvirus,five degenerated primers were designedThey were Sprimer(5′-GGX AAY AAY AGY GGX CAZ CC-3′),pNIa(+)(5′-TNY TGG AAM CAY TGG AT-3′),pCI2(+)(5′-GCX ACX AAX ATX ATX GAX AA-3′),pCI1(+)(5′-GTX GGX TCX GGX AAX TCX AC-3′)and pHC(+)(5′-TGY GAY AAY CAZ TTX GA-3′)(X=A,T,C or G:Y=T or C;Z=A or G;N=A or T;M=A,T or G)Using degenerated PCR and modified RACE methods,a protocol for determination of complete genome sequence of potyviruses was established and proved to be successful on fiveBased on multi-alignment of complete polyprotein amino acid sequences of genus Potyvirus,five degenerated primers were designedThey were Sprimer(5′-GGX AAY AAY AGY GGX CAZ CC-3′),pNIa(+)(5′-TNY TGG AAM CAY TGG AT-3′),pCI2(+)(5′-GCX ACX AAX ATX ATX GAX AA-3′),pCI1(+)(5′-GTX GGX TCX GGX AAX TCX AC-3′)and pHC(+)(5′-TGY GAY AAY CAZ TTX GA-3′)(X=A,T,C or G:Y=T or C;Z=A or G;N=A or T;M=A,T or G)Using degenerated PCR and modified RACE methods,a protocol for determination of complete genome sequence of potyviruses was established and proved to be successful on five potyviruses

关 键 词：Potyvirus属成员 基因组分序列 PCR RACE 扩增方法 马铃薯Y病毒属 简并引物 全序列测定 扩增方法 

分 类 号：S432.41[农业科学—植物病理学]