痛风肾泰方含药血清逆转非结晶尿酸导致HK-2细胞间质转分化的体外实验研究  

Vitro Experimental Study of Serum Containing Tongfeng Shentai Recipe(痛风肾泰方)Reversing Epithelial-Mesenchymal Transition of HK-2 Cells Caused by Non-crystalline Uric Acid

作　　者：薄敏敏 吴沅皞[2] 曹克光[2] BO Minmin;WU Yuanhao;CAO Keguang(Beijing Chaoyang District Hospital of Traditional Chinese Medicine,Beijing 100020,China;The First Affiliated Hospital of Tianjin University of Traditional Chinese Medicine,Tianjin 300073,China)

机构地区：[1]北京市朝阳区中医医院,北京100020 [2]天津中医药大学第一附属医院,天津300073

出　　处：《辽宁中医杂志》2022年第11期192-195,共4页Liaoning Journal of Traditional Chinese Medicine

基　　金：国家自然科学基金(81673927)。

摘　　要：目的 探讨痛风肾泰方逆转非结晶尿酸导致的人肾小管上皮细胞转分化的作用机理。方法 采用体外非结晶尿酸培养HK-2细胞造模,痛风肾泰方大鼠灌胃制备含药血清,采用放射免疫法检测培养液内生长转化因子-β1(TGF-β1)浓度,RT-PCR检测HK-2细胞TGFβRImRNA、TGFβRIImRNA、α平滑肌肌动蛋白(α-SMA)mRNA、E-cadherin mRNA、Smad3mRNA、Smad7mRNA含量。结果 与对照组比较,尿酸组TGF-β1浓度、TGFβRImRNA、α-SMA mRNA、Smad3 mRNA升高、E-cadherin mRNA降低,具有统计学意义,TGFβRIImRNA、Smad7轻度升高,结果无统计学意义;与尿酸组比较,中药组TGF-β1浓度、α-SMA mRNA、Smad3 mRNA降低,具有统计学意义,E-cadherin升高、TGFβRI、TGFβRIImRNA降低,无统计学意义,Smad7具有明显的升高作用,与对照组比较差异有统计学意义。结论 非结晶尿酸可以通过TGF-β1-TGFβR-Smad途径导致HK-2细胞EMT,中药痛风肾泰方含药血清具有抑制该通路、逆转HK-2细胞EMT发生的作用。Objective To investigate the mechanism of Tongfeng Shentai Recipe(痛风肾泰方)reversing human renal tubule epithelial cells epithelial-mesenchymal transition(EMT)caused by non-crystallized uric acid.Methods HK-2 cells were cultured with non-crystallized uric acid in vitro,and the drug-containing serum was prepared by intragastric administration in rats with Tongfeng Shentai Recipe.The contents of transforming growth factor-β1(TGF-β1)in the medium was detected by radioimmunoassay.The mRNA contents of TGFβRImRNA,TGFβRIImRNA,αsmooth muscle actin(α-SMA)mRNA,E-cadherin mRNA,Smad3 mRNA and Smad7 mRNA in HK-2 cells were detected by RT-PCR.Results Compared with those of the control group,the contents of TGF-β1,TGFβRImRNA,α-SMA mRNA,Smad3 mRNA in uric acid group were increased and the content of E-cadherin was decreased,with statistical significance.TGFβRIImRNA and Smad7 were slightly increased,and the results were not statistically significant.Compared with those of the uric acid group,the contents of TGF-β1,α-SMA mRNA and Smad3 mRNA in Tongfeng Shentai Recipe group were decreased with statistical significance.The content of E-cadherin was increased and the contents of TGFβRI and TGFβRIImRNA were decreased without statistical significance.Compared with those of the control group,Smad7 had a significant elevation.Conclusion Non-crystalline uric acid can induce EMT in HK-2 cells through TGF-β1-TGFβR-Smad pathway,and the serum containing Tongfeng Shentai Recipe can inhibit this pathway and reverse EMT of HK-2 cells.

关 键 词：痛风肾泰方 非结晶尿酸 肾小管上皮细胞转分化 TGF-β1-TGFβR-Smad通路 

分 类 号：R971.1[医药卫生—药品]