不同剂量链脲佐菌素对糖尿病肾病病证结合动物模型证候类型影响的研究  

Effects of Different Doses STZ on Syndrome Types of Diabetic Nephropathy Combined with Animal Models

作　　者：戴思瑶 王丽[2] DAI Siyao;WANG Li(Liaoning University of Traditional Chinese Medicine,Shenyang 110847,Liaoning,China;Affiliated Hospital of Liaoning University of Traditional Chinese Medicine,Shenyang 110032,Liaoning,China)

机构地区：[1]辽宁中医药大学,辽宁沈阳110847 [2]辽宁中医药大学附属医院,辽宁沈阳110032

出　　处：《辽宁中医药大学学报》2022年第12期36-39,F0003,共5页Journal of Liaoning University of Traditional Chinese Medicine

基　　金：辽宁省自然科学基金指导计划项目(2018011148-201);沈阳市科技计划项目(19-112-4-077)。

摘　　要：目的通过注射不同剂量的链脲佐菌素(STZ)建立糖尿病肾病大鼠模型,对比各剂量组大鼠在肾脏生化指标、病理形态改变以及出现的症状、体征等方面的差异,从而对不同剂量制备糖尿病肾病大鼠“病证结合”动物模型的证候类型进行探讨。方法按大鼠体质量将40只SD雄性大鼠分为对照组(control组)及模型组(A组、B组、C组)。分别对A、B、C组大鼠进行腹腔注射STZ溶液50、60、70 mg/kg体质量。control组大鼠予2 mL生理盐水以相同方式注射。造模第6周末检测大鼠空腹血糖、尿微量白蛋白(mALB)、尿素氮(BUN)、血肌酐(Scr),观察肾脏病理。通过各组大鼠第6周的症状、体征等表现判断其所属证候类型。结果B组与C组大鼠在肾脏生化指标上的损害并无明显区别,但两组mALB、BUN、Scr水平均显著高于A组大鼠。光镜下观察大鼠肾脏病理形态结果为C组大鼠肾脏病理损害较B组稍加显著。从大鼠症状表现上看,A组大鼠以单纯津伤症状为主,尚未累及脏腑。随着造模剂量的增加,B组大鼠出现以脾肾气虚为主的脏腑虚损表现。C组大鼠则出现脏腑、津液亏虚的整体虚损表现。结论腹腔注射50、60、70 mg/kg的STZ剂量6周后,各组大鼠分别表现为阴津亏虚证、脾肾气虚证、气阴两虚证。Objective To establish diabetic nephropathy rat model by injecting different doses of streptozotocin(STZ),and compare the differences in renal biochemical indexes,pathological changes,symptoms and signs of rats in different dose groups,so as to explore the syndrome types of animal models of diabetic nephropathy treated by different doses.Methods Forty male SD rats were divided into control group and model group according to their body mass.Rats in groups A,B and C were injected intraperitoneally with STZ solution of 50 mg/kg,60 mg/kg and 70 mg/kg respectively.Rats in con group were injected with 2m L normal saline in the same way.At the end of the 6th week of modeling,the indexes of renal damage such as fasting blood glucose,urinary microalbumin(mALB),urea nitrogencB(BUN)and serum creatinine(Scr)were measured,and the renal pathology was observed.The syndrome types of rats in each group were judged by their symptoms and signs in the sixth week.Results There was no significant difference in renal biochemical indexes between group B and group C,but the renal damage indexes of the two groups were significantly higher than those of group A.The pathological morphology of rat kidney was observed under light microscope.The results showed that the pathological damage of kidney in group C was slightly more significant than that in group B.From the aspect of rat symptoms,the rats in group A mainly suffered from simple fluid injury,and the viscera were not involved.With the increase of modeling dose,the rats in group B showed the deficiency of spleen and kidney Qi,mainly the deficiency of viscera.The rats in group C showed the overall deficiency of viscera and body fluid.Conclusion Six weeks after intraperitoneal injection of 50 mg/kg,60 mg/kg and 70 mg/kg STZ,rats in each group showed fluid deficiency syndrome,spleen and kidney Qi deficiency syndrome and Qi Yin deficiency syndrome respectively.

关 键 词：糖尿病肾病 SD大鼠 病证结合动物模型 

分 类 号：R587.2[医药卫生—内分泌]