M3受体遗传变异与血压钠钾反应性的关联研究  

Associations of genetic variants in M3 receptor with blood pressure responses to salt and potassium intake

作　　者：张玺 胡桂霖 牛泽家馨 杜鸣飞 邹婷 张晓玉 王兰 褚超[1] 廖月圆 马琼[1] 王丹[1] 王科科 贾昊 陈晨[1] 严瑜 孙月 郭统帅[1] 张婕 高卫华[5] 闫睿晨 高可 罗文婧 牟建军[1] 汪洋[1,6] ZHANG Xi;HU Guilin;NIU Zejiaxin;DU Mingfei;ZOU Ting;ZHANG Xiaoyu;WANG Lan;CHU Chao;LIAO Yueyuan;MA Qiong;WANG Dan;WANG Keke;JIA Hao;CHEN Chen;YAN Yu;SUN Yue;GUO Tongshuai;ZHANG Jie;GAO Weihua;YAN Ruichen;GAO Ke;LUO Wenjing;MU Jianjun;WANG Yang(Department of Cardiovascular Medicine,The First Affiliated Hospital of Xi’an Jiaotong University,Xi’an 710061;Department of Cardiology,Northwest Women’s and Children’s Hospital,Xi’an 710003;Department of Cardiology,Xi’an International Medical Center Hospital,Xi’an 710100;Department of Cardiology,Xi’an People’s Hospital,Xi’an 710004;Department of Cardiology,Xi’an No.1 Hospital,Xi’an 710002;Global Health Institute,Xi’an Jiaotong University,Xi’an 710061,China)

机构地区：[1]西安交通大学第一附属医院心内科,陕西西安710061 [2]西北妇女儿童医院心内科,陕西西安710003 [3]西安国际医学中心医院心内科,陕西西安710100 [4]西安市人民医院心内科,陕西西安710004 [5]西安市第一医院心内科,陕西西安710002 [6]西安交通大学全球健康研究院,陕西西安710061

出　　处：《西安交通大学学报（医学版）》2023年第1期46-54,共9页Journal of Xi’an Jiaotong University（Medical Sciences）

基　　金：国家自然科学基金资助项目(No.81600327,81870319,82070437);陕西省自然科学基金项目(No.2021JM-257,2021JM-588);西安交通大学第一附属医院院基金(No.2019QN-06、2021ZXY-14)资助;西安交通大学第一附属医院临床研究项目(No.XJTU1AF-CRF-2019-004、XJTU1AF2021CRF-021)。

摘　　要：目的M3毒蕈碱乙酰胆碱受体(M3受体)由CHRM3基因编码,广泛分布于心血管系统并发挥重要的心脏调节作用。本研究旨在探讨M3受体遗传变异与钠钾饮食干预后血压反应性之间的关系。方法从中国陕西省农村地区招募来自124个家庭的333名受试者,经过3 d的基线观察后,依次进行7 d低盐饮食、7 d高盐饮食、7 d高盐补钾饮食。选取13个CHRM3基因单核苷酸多态性(SNP)位点进行相关分析。结果CHRM3基因SNP位点rs10802811与低盐期及高盐期舒张压及平均动脉压反应性显著相关,而rs6429147、rs373288072、rs114677844、rs663148与高盐期收缩压及平均动脉压反应性显著相关。此外,rs6692904与高盐补钾期收缩压、舒张压及平均动脉压反应性显著相关。结论M3受体基因多态性与血压钠钾反应性相关,提示M3受体基因可能参与血压盐敏感性及钾敏感性的形成。Objective M3 muscarinic acetylcholine receptor(M3 receptor),encoded by CHRM3 gene,is widely distributed in the cardiovascular system and plays an important role in cardiac regulation.The aim of this study was to assess the association of genetic variants in M3 receptor with blood pressure(BP)responses to controlled dietary sodium and potassium interventions.Methods A total of 333 subjects from124 families were recruited from the rural areas of northern China.After a three-day baseline observation,they were sequentially on a sevenday low-salt diet,a seven-day high-salt diet,and a seven-day high-salt diet plus potassium supplementation.Thirteen CHRM3 single nucleotide polymorphisms(SNPs)were selected for analysis.Results SNP rs10802811 of the CHRM3 was significantly associated with diastolic BP(DBP)and mean arterial pressure(MAP)responses to both low-salt and high-salt diets while SNPs rs6429147,rs373288072,rs114677844 and rs663148 showed significant associations with systolic BP(SBP)and MAP responses to high-salt diet.In addition,SNP rs6692904 was significantly associated with SBP,DBP and MAP responses to high-salt diet with potassium supplementation.Conclusion Genetic variants in M3 receptor are significantly associated with BP responses to sodium and potassium intervention,suggesting that M3 receptor may be mechanistically involved in BP salt and potassium sensitivity.

关 键 词：M3毒蕈碱乙酰胆碱受体 基因多态性 血压 盐 钾 

分 类 号：R541.3[医药卫生—心血管疾病]