2型糖尿病合并白内障患者晶状体上皮细胞中PEDF和VEGF的表达及意义  

Expression and significance of pigment epithelium-derived factor and vascular endothelial growth factor in lens epithelial cells of diabetic patients with cataract

作　　者：胡亚茹 陈悦 韩雪 陈亚茹 马竹宇 王剑锋[1] Ya-Ru Hu;Yue Chen;Xue Han;Ya-Ru Chen;Zhu-Yu Ma;Jian-Feng Wang(Department of Ophthalmology,the First Affiliated Hospital of Bengbu Medical College,Bengbu 233004,Anhui Province,China;Bengbu Medical College,Bengbu 233030,Anhui Province,China)

机构地区：[1]蚌埠医学院第一附属医院眼科,安徽省蚌埠市233004 [2]蚌埠医学院,安徽省蚌埠市233030

出　　处：《国际眼科杂志》2023年第1期109-112,共4页International Eye Science

基　　金：安徽省大学生创新创业计划项目(No.S202010367029);蚌埠医学院科技项目(No.2020byzd141)。

摘　　要：目的:观察糖尿病合并年龄相关性白内障患者晶状体上皮细胞(LECs)中色素上皮衍生因子(PEDF)、血管内皮细胞生长因子(VEGF)的表达水平,探讨糖尿病合并年龄相关性白内障的发病机制。方法:回顾性研究。收集2020-08/2021-04在蚌埠医学院第一附属医院眼科就诊的年龄相关性白内障和2型糖尿病合并年龄相关性白内障患者各30例。所有患者白内障超声乳化术中收取术眼晶状体中央区5.5~6.0mm直径的前囊膜标本,采用蛋白免疫印迹法(Western-blot)检测LECs中PEDF、VEGF蛋白表达水平。实时荧光定量PCR(qRT-PCR)方法检测PEDF、VEGF mRNA的相对表达量。结果:两组患者LECs中均存在PEDF、VEGF的表达,2型糖尿病合并年龄相关性白内障组患者VEGF mRNA相对表达量为1.364±0.062,高于年龄相关性白内障组的1.000±0.0(P<0.01),PEDF mRNA的相对表达量为0.398±0.053,明显低于年龄相关性白内障组的1.000±0.0(P<0.001)。2型糖尿病合并年龄相关性白内障组患者LECs中VEGF和PEDF蛋白表达量为2.053±0.026、0.579±0.045,年龄相关性白内障组为1.680±0.064、1.058±0.007(均P<0.01)。结论:2型糖尿病合并年龄相关性白内障患者LECs中PEDF和VEGF表达水平发生变化,可能与糖尿病患者白内障的发生和发展有关。·AIM: To observe the expression levels of pigment epithelium-derived factor(PEDF) and vascular endothelial growth factor(VEGF) in lens epithelial cells(LECs) of diabetic patients with age-related cataract, and explore the pathogenesis of diabetic with age-related cataract.·METHODS: Retrospectively study. A total of 30 patients with age-related cataract(ARC group) and 30 patients with type 2 diabetes mellitus combined with age-related cataract(DC group) who treated at the Department of Ophthalmology of the First Affiliated Hospital of Bengbu Medical College from August 2020 to April 2021 were collected. Anterior capsule specimens with a diameter of 5.5-6.0 mm in the central region of the intraoperative eye were collected during phacoemulsification. The protein expression levels of PEDF and VEGF in LECs were detected by Western-blot. Quantitative real-time PCR(qRT-PCR) was used to detect the relative mRNA expression levels of PEDF and VEGF.·RESULTS:PEDF and VEGF were expressed in LECs of both groups, and the relative expression of VEGF mRNA in DC group was 1.364±0.062, which was higher than that of ARC group(1.000±0.0;P<0.01). The relative mRNA expression level of PEDF was 0.398±0.053, which was significantly lower than 1.000±0.0 in the ARC group(P<0.001). The expression of VEGF protein in LECs was 2.053±0.026 in DC group and 1.680±0.064 in ARC group, respectively. Meanwhile, the expression of PEDF protein was 0.579±0.045 in DC group and 1.058±0.007 in ARC group(all P<0.01).·CONCLUSION:The changes of PEDF and VEGF expression levels in LECs may be related to the occurrence and development of cataract in diabetic patients.

关 键 词：糖尿病合并白内障 晶状体上皮细胞(LECs) 色素上皮衍生因子(PEDF) 血管内皮细胞生长因子(VEGF) 

分 类 号：R587.2[医药卫生—内分泌] R776.1[医药卫生—内科学]