超分辨显微成像技术在细胞器相互作用研究中的应用(特邀)  

Application of super-resolution microscopy in the study of organelle interactions(invited)

作　　者：戴太强 高晔 马英 蔡卜磊 刘富伟 何博聆 禹洁 侯燕 郜鹏 孔亮 Dai Taiqiang;Gao Ye;Ma Ying;Cai Bolei;Liu Fuwei;He Boling;Yu Jie;Hou Yan;Gao Peng;Kong Liang(State Key Laboratory of Military Stomatology,Xi’an 710032,China;National Clinical Research Center for Oral Diseases,Xi’an 710032,China;Shaanxi Clinical Research Center for Oral Diseases,Xi’an 710032,China;Department of Oral and Maxillofacial Surgery,School of Stomatology,The Fourth Military Medical University,Xi’an 710032,China;School of Physics,Xidian University,Xi’an 710171,China)

机构地区：[1]军事口腔医学国家重点实验室,陕西西安710032 [2]国家口腔疾病临床医学研究中心,陕西西安710032 [3]陕西省口腔疾病临床医学研究中心,陕西西安710032 [4]第四军医大学口腔医院颌面外科,陕西西安710032 [5]西安电子科技大学物理学院,陕西西安710171

出　　处：《红外与激光工程》2022年第11期48-59,共12页Infrared and Laser Engineering

基　　金：国家重点研发计划政府间国际科技创新合作(2022YFE0100700);空军军医大学伙伴实验室合作交流计划(2022HB011);国家自然科学基金(82101065)。

摘　　要：观察细胞器间动态相互作用,深入分析作用规律,对于揭示生理病理过程现象背后的机制具有十分重要的意义。传统光学显微镜受到由光波波长和孔径造成的衍射极限的限制,无法观测细胞器纳米级精细结构及细胞器间相互作用的动态变化规律。超分辨显微成像技术的出现为细胞器相互作用研究提供了重要手段,在深入揭示细胞器相互作用规律,阐明生理病理现象深层的机制研究中发挥了重要的作用。文中介绍了受激发射损耗(Stimulated emission depletion,STED)显微成像、结构光照明显微成像(Structured illumination microscopy,SIM)、单分子定位显微成像(Single molecule localization microscopy,SMLM)技术,并总结了这三类超分辨显微成像技术在细胞器相互作用中的应用与现状,为超分辨显微成像技术在细胞器相互作用研究中的应用提供思路拓展。最后,对超分辨显微成像技术在细胞器相互作用研究中的优势与不足进行分析总结,展望了超分辨显微成像技术在活细胞内细胞器相互作用成像中的需求发展趋势,为光学与医学及生物学的交叉融合发展提供一定的参考。Observing dynamic interaction between organelles and analyzing the law of action is of great significance for revealing the mechanism behind the phenomenon of physiological and pathological processes.Due to the limitation of the optical diffraction determined by wavelength and aperture,traditional optical microscopes cannot observe the nanoscale fine structure of organelles and the dynamic changes of interactions among them.The emergence of super-resolution microscopy imaging technology provides an important mean for the study of organelle interaction.This paper introduces the fluorescence microscopy(STED),structured illumination imaging(SIM),and single-molecule localization imaging(SMLM).The application of these superresolution microscopy in the study of dynamic interaction between organelles provides the expansion of application ideas for super-resolution microscopy.Finally,the advantages and disadvantages of super-resolution microscopy in the study of organelle interactions are analyzed.In conclusion,the demand and development trend of super-resolution microscopy technology in the imaging of intracellular organelle interaction in living cells is prospected,which provides a certain reference for the cross-integration development of optics,medicine and biology.

关 键 词：超分辨显微成像 细胞器间相互作用 受激发射损耗显微成像 结构光照明显微成像 单分子定位显微成像 

分 类 号：O439[机械工程—光学工程] Q63[理学—光学]