中国野生猕猴桃资源的溃疡病抗性种质筛选  

Screening of wild Actinidia germplasms resistant to bacterial canker disease in China

作　　者：李黎[1] 潘慧[1] 李文艺 汪祖鹏 钟彩虹[1] Li Li;Pan Hui;Li Wen-Yi;Wang Zu-Peng;Zhong Cai-Hong(Engineering Laboratory for Kiwifruit Industrial Technology,Key Laboratory of Plant Germplasm Enhancement and Specialty Agriculture,Wuhan Botanical Garden,Chinese Academy of Sciences,Wuhan 430074,China)

机构地区：[1]中国科学院猕猴桃产业技术工程实验室,中国科学院武汉植物园植物种质创新与特色农业重点实验室,武汉430074

出　　处：《植物科学学报》2022年第6期801-809,共9页Plant Science Journal

基　　金：国家重点研发计划(2019YFD1000201);湖北省重点研发计划(2022BBA0076);植物基因组学国家重点实验室开放课题(SKLPG2016A-18);长江猕猴桃产业技术研究项目(CJZX20210102);财政部和农业农村部:国家现代农业产业技术体系项目(CARS-26)。

摘　　要：细菌性溃疡病严重威胁猕猴桃(Actinidia)产业发展,本研究对国家猕猴桃资源圃中29个种82份种质资源进行了离体枝条的溃疡病菌接种,以病斑长度评价其抗病能力,将种质划分为高抗至高感7个抗性等级。结果显示:不同种间材料抗性差异显著,大部分种内材料存在明显的抗性分化。82份种质中含高抗种质5份(6.09%)、中抗种质9份(10.98%)、低抗种质18份(21.95%)、耐病种质21份(25.61%)、低感种质11份(13.41%)、中感种质9份(10.98%)及高感种质9份(10.98%)。32份抗病种质包括软枣(A.arguta(Siebold&Zuccarini)Planchon ex Miquel)6份,异色(A.callosa var.discolor C.F.Liang)5份,黑蕊(A.melanandra Franchet)4份,京梨(A.callosa var.henryi Maximowicz)及毛花(A.eriantha Bentham)各3份,葡萄叶(A.vitifolia C.Y.Wu)、安息香(A.styracifolia C.F.Liang)及紫果(A.arguta(Siebold&Zuccarini)Planchon ex Miquel var.purpurea(Rehd.)C.F.Liang)各2份,阔叶(A.latifolia(Gardner&Champion)Merrill)、红茎(A.rubricaulis Dunn)、山梨(A.rufa(Siebold&Zuccarini)Planchon ex Miquel)、湖北(A.hubeiensis H.M.Sun and R.H.Huang)及黄毛(A.fulvicoma Hance)各1份。本研究结果为猕猴桃抗病品种的杂交选育与创制奠定了基础。Bacterial canker disease is a serious threat to the development of the kiwifruit industry. Based on the abundant wild Actinidia germplasm resources in China, screening highly resistant germplasms and breeding resistant varieties with great fruit characters is a primary task for the development of the kiwifruit industry. In this paper, we inoculated isolated branches of 82 germplasms of 29 Actinidia species from the national kiwifruit germplasm nursery with Pseudomonas syringae pv. actinidiae. Disease resistance was evaluated based on lesion length, divided into seven categories from highly resistant to highly susceptible. Results showed distinct differences in resistance among the different species, as well as intraspecific resistance differentiation. Among the 82 germplasms, five were highly resistant(6.09%), nine were moderately resistant(10.98%), 18 were lowly resistant(21.95%), 21 were tolerant(25.61%), 11 were lowly susceptible(13.41%), nine were moderately susceptible(10.98%), and nine were highly susceptible(10.98%). The 32 resistant germplasms included six germplasms of A. arguta(Siebold & Zuccarini) Planchon ex Miquel, five germplasms of A. callosa var. discolor C. F. Liang, four germplasms of A. melanandra Franchet, three germplasms of A. callosa var. henryi Maximowicz and A. eriantha Bentham, respectively, two germplasms of A. vitifolia C. Y. Wu, A. styracifolia C. F. Liang, and A. arguta(Siebold & Zuccarini) Planchon ex Miquel var. purpurea(Rehd.) C. F. Liang, respectively, and one germplasm of A. latifolia(Gardner & Champion) Merrill, A. rubricaulis Dunn, A. rufa(Siebold & Zuccarini) Planchon ex Miquel, A. hubeiensis H. M. Sun and R. H. Huang, and A. fulvicoma Hance, respectively. Thus, this study lays a theoretical foundation for the cross-breeding and creation of disease-resistant varieties of kiwifruit.

关 键 词：猕猴桃 溃疡病 种质资源 离体分析 抗性评价 

分 类 号：S663.4[农业科学—果树学]