不同传染性法氏囊病病毒株感染对CCL19-CCR7轴系表达量的影响  

Effects of Different Infectious Bursal Disease Virus Strains on CCL19-CCR7 Axis Expression

作　　者：王秋霞[1] 张新 楚富茗 王明明 王芳[1] 卢浪 魏小兵[1] 余燕[1] 张艳红[1] 马金友[1] 姜金庆[1] 欧长波[1] 刘兴友[1,2] WANG Qiu-xia;ZHANG Xin;CHU Fu-ming;WANG Ming-ming;WANG Fang;LU Lang;WEI Xiao-bing;YU Yan;ZHANG Yan-hong;MA Jin-you;JIANG Jin-qing;OU Chang-bo;LIU Xing-you(College of Animal Sciences and Veterinary Medicine,Henan Institute of Science and Technology,Xinxiang 453003,China;School of Life Science&Basic Medicine,Xinxiang University,Xinxiang 453003,China)

机构地区：[1]河南科技学院动物科技学院,河南新乡453003 [2]新乡学院生命科学与基础医学学院,河南新乡453003

出　　处：《中国兽医杂志》2022年第11期27-32,共6页Chinese Journal of Veterinary Medicine

基　　金：河南省科技攻关项目(202102110254,212102110009);河南省高等学校重点科研项目(21A230003);河南省高校科技创新团队支持计划(20IRTSTHN025)。

摘　　要：为了解不同毒株传染性法氏囊病病毒(IBDV)对CC类趋化因子配体CCL19及其受体CCR7基因表达量的影响,探明趋化因子在IBDV致病中的作用,本试验分别用经典毒株CJ801和本地分离毒株HN-1对SPF鸡进行攻毒,攻毒成功后取法氏囊提取总RNA,反转录后荧光定量分析,检测CCL19-CCR7轴系基因mRNA表达水平的差异。结果显示,经典毒株CJ801攻毒后,趋化因子CCL19及其受体CCR7基因mRNA表达量均于1d时达峰值,3d时显著下降低;而本地分离毒株HN-1攻毒后,趋化因子CCL19及其受体CCR7基因mRNA表达量于1d、3d和5d逐步上升。结果表明,在IBDV经典毒株CJ801和本地分离毒株HN-1感染过程中,CCL19及其受体CCR7基因表达量差异显著,其对T细胞迁移和IBDV复制的影响机制有待进一步探索。This study investigated the effects of different strains of infectious bursal disease virus(IBDV) on the gene expression levels of CC chemokine ligand CCL19 and its receptor CCR7. Classical and local isolated IBDV strains(CJ801 and HN-1) were used to infect SPF chickens. After IBDV challenge, the total RNA was extracted from the bursa, and the mRNA expression levels of CCL19-CCR7 was assessed by quantitative reverse transcription PCR. The results showed that under classical IBDV strain CJ801 challenge, the mRNA expression levels of chemokine CCL19 and its receptor CCR7 peaked after day 1 and declined after day 3, whereas under local isolated IBDV strain HN-1 challenge, the mRNA expression levels of chemokine CCL19 and its receptor CCR7 gradually increased from day 1, day 3 to day 5. Thus, classical and local isolated IBDV strains(CJ801 and HN-1) appear to have contrasting impact on CCL19 and its receptor CCR7 expression, and further study is necessary to clarify the role of CCL19 and its receptor CCR7 in T cells migration or IBDV replication.

关 键 词：趋化因子 趋化因子受体 CCL19 CCR7 表达量 

分 类 号：S855.3[农业科学—临床兽医学]