仔猪细菌性腹泻病原菌的分离鉴定与耐药性分析  

Isolation, Identification and Drug Resistance Analysis of Pathogenic Bacteria in Bacterial Diarrhea Piglets

作　　者：吴杨 闫艳新 孙虎芝 潘强 任慧英 张灿[3] WU Yang;YAN Yan-xin;SUN Hu-zhi;PAN Qiang;REN Hui-ying;ZHANG Can(Qingdao Phageparm Bio-tech Co.,Ltd.,Qingdao 266000,China;Qingdao Special Food Research Institute,Qingdao 266109,China;College of Veterinary Medicine,Qingdao Agricultural University,Qingdao 266109,China)

机构地区：[1]青岛诺安百特生物技术有限公司,山东青岛266000 [2]青岛特种食品研究院,山东青岛266109 [3]青岛农业大学动物医学院,山东青岛266109

出　　处：《中国兽医杂志》2022年第11期95-99,共5页Chinese Journal of Veterinary Medicine

基　　金：山东省重点研发计划(2019GNC106108);山东省重点研发计划项目(2019 JZZY010720)。

摘　　要：为了解山东省仔猪细菌性腹泻的病原情况,本试验于2019年1月—2020年5月在山东青岛、烟台和潍坊地区采集了1 014份仔猪腹泻样品,进行了病原菌的分离鉴定,并对分离到的大肠杆菌进行毒力基因鉴定及药敏试验。结果显示:常见仔猪腹泻致病菌大肠杆菌、沙门菌和产气荚膜梭菌的分离率分别为97.34%(987/1 014)、3.45%(35/1 014)和0.39%(4/1 014)。致病性大肠杆菌中黏附素的主要类型为K88(47.34%)和F18ac(31.95%),毒素类型主要为LT1(48.52%)和ST2(31.36%)。对169株致病性大肠杆菌的药敏试验结果显示,超过50%的大肠杆菌具有强耐药性。本试验结果为仔猪细菌性腹泻的预防及治疗提供参考和依据。In order to determine the causes of piglet bacterial diarrhea in Shandong Province, this study collected 1 014 piglet diarrhea samples from Qingdao, Yantai and Weifang district for bacterial isolation and identification, and examined virulence genes and drug susceptibility of the resulting Escherichia coli isolates. The results showed that the isolation rates of E. coli,Salmonella and Clostridium perfringens were 97.34%(987/1 014),3.45%(35/1 014) and 0.39%(4/1 014), respectively;the main types of adhesin were K88(47.34%) and F18 ac(31.95%);and the main types of toxin were LT1(48.52%) and ST2(31.36%). The drug susceptibility test of 169 pathogenic E.coli strains showed that more than 50% had strong drug resistance. This study provides valuable data for the prevention and treatment of piglet bacterial diarrhea.

关 键 词：仔猪 腹泻 大肠杆菌 毒力基因 耐药性 

分 类 号：S852.61[农业科学—基础兽医学]