藏猪和大约克夏猪CXCL10基因多态性及表达差异分析  

Analysis of CXCL10 Gene Polymorphism and Expression Differences between Tibetan and Yorkshire Pigs

作　　者：刘思源 何辰庆 陈军军 叶幼荣 张健 商鹏 强巴央宗 LIU Siyuan;HE Chenqing;CHEN Junjun;YE Yourong;ZHANG Jian;SHANG Peng;Yangzom Chamba(College of Animal Science,Tibet Agricultural and Animal Husbandry College,Tibet Linzhi 860000,China;Provincial-Ministry Collaborative Innovation Center for Research and Development of Tibetan Special Agricultural and Animal Husbandry Resources,Tibet Linzhi 860000,China)

机构地区：[1]西藏农牧学院动物科学学院,西藏林芝860000 [2]西藏特色农牧资源研发省部共建协同创新中心,西藏林芝860000

出　　处：《中国畜牧杂志》2022年第11期100-104,共5页Chinese Journal of Animal Science

基　　金：西藏自治区重大科技专项项目(XZ202101ZD0005N);中国农业大学基本科研业务费专项资金项目(2021TC002);西藏农牧学院研究生教育创新计划项目(YJS2020-14)。

摘　　要：为探讨CXCL10基因在藏猪和大约克夏猪中的差异表达和多态性分析,本研究采用一代测序(Sanger测序)法对藏猪和大约克夏猪CXCL10基因起始密码子上游3 kb区域进行单核苷酸多态性(Single Nucleotide Polymorphism,SNP)筛选并进行基因分型;采用RT-qPCR检测CXCL10基因在180日龄藏猪和大约克夏猪背最长肌、腿肌、肝脏组织中的mRNA水平表达情况。结果显示:在CXCL10基因起始密码子上游3 kb区域,存在T-832G、A-808G和T-694C这3个品种间极显著差异的SNPs位点,且均符合哈迪-温伯格定律;转录因子预测结果显示,这些SNPs位点的突变导致了ARGFX、ARID3A/B的结合位点消失;CXCL10基因在藏猪背最长肌、腿肌和肝脏组织中的表达量均极显著高于大约克夏猪。由此推测,CXCL10基因可能参与肌肉生长发育的负向调控。本研究结果为进一步研究藏猪生长发育机制奠定了基础。To investigate the differential expression and polymorphism analysis of CXCL10 gene in Tibetan and Yorkshire pigs, Single Nucleotide Polymorphisms(SNPs) were screened and genotyped in the 3 kb region upstream of the CXCL10 gene start codon in Tibetan and Yorkshire pigs by generation sequencing(Sanger sequencing);RT-qPCR was used to detect the expression of CXCL10 gene in 180-day-old Tibetan and Yorkshire pigs. The expression of CXCL10 gene in the longest dorsal muscle, leg muscle and liver tissues of 180-day-old Tibetan and Yorkshire pigs was examined by RT-qPCR. The results showed that in the 3 kb upstream region of the CXCL10 gene start codon, there were SNPs sites with highly significant differences among the three breeds, T-832G, A-808G and T-694C(P<0.01), and all of them conformed to the Hardy-Weinberg law;the transcription factor prediction results showed that mutations in these SNPs sites resulted in the loss of the ARGFX,ARID3A/B binding site. The expression of CXCL10 gene in the longest dorsal muscle, leg muscle and liver tissues of Tibetan pigs was highly significant higher than that of Yorkshire pigs(P<0.01). It was hypothesized that CXCL10 gene might be involved in the negative regulation of muscle growth and development, laying the foundation for further studies on the growth and development mechanism of Tibetan pigs.

关 键 词：猪 CXCL10基因 MRNA表达 多态性 

分 类 号：S828.2[农业科学—畜牧学]