中国荷斯坦牛乳房炎抗性相关SNP效应扩群验证及分析  

Genetic Effect of SNP Loci Associated with Mastitis Resistance in an Extended Validation Cohort of Chinese Holstein Cattle

作　　者：周月玲 达日格日乐 唐永杰 李文龙 文惠 赵婷婷 肖炜 俞英[1] Zhou Yueling;Darigerile;Tang Yongjie;Li Wenlong;Wen Hui;Zhao Tingting;Xiao Wei;Yu Ying(Department of Animal Breeding and Genetics,College of Animal Sciences and Technology,China Agricultural University,Beijing100193,China;Beijing SunLon Livestock Development Co.,Ltd,Beijing 100076,China;Beijing Animal Husbandry Station,Beijing 100107,China)

机构地区：[1]中国农业大学动物科学技术学院,北京100193 [2]北京首农畜牧发展有限公司,北京100076 [3]北京市畜牧总站,北京100107

出　　处：《中国畜牧杂志》2022年第12期92-98,共7页Chinese Journal of Animal Science

基　　金：国家自然科学基金国际(地区)合作项目(31961143009);北京首农畜牧发展有限公司自立科研课题申请书重点专项(SYZYZ20190003);北京市奶牛产业创新团队(BAIC06);北京市自然科学基金(6182021);财政部和农业农村部:现代农业产业技术体系项目(CARS-36)。

摘　　要：奶牛乳房炎对奶业影响巨大。课题组前期通过全基因组关联分析(GWAS)筛选到奶牛乳房炎抗性相关基因(TRAPPC9、CD4、DGAT1)的共4个单核苷酸多态性标记(SNPs),本研究旨在扩大群体验证上述SNPs在5个不同的中国荷斯坦牛群体中与乳房炎的相关性。实验采集了来自5个奶牛群体的共895头中国荷斯坦牛血样并提取DNA用于SNP分型,利用分型结果和2020年DHI数据建立一般线性模型,分析SNPs对乳房炎抗性和产奶性状的遗传效应。结果表明,SNP1、SNP2、SNP3和SNP4对中国荷斯坦牛体细胞数或体细胞评分的遗传效应在多个月显著。同时,还发现SNP1和SNP4对中国荷斯坦牛305 d产奶量的遗传效应在多个月显著。本研究表明,在中国荷斯坦牛群体中,SNP1、SNP2、SNP3和SNP4与奶牛乳房炎抗性有关,并且TRAPPC9、CD4和DGAT1可以作为中国荷斯坦牛乳房炎抗性的分子标记,为中国荷斯坦牛乳房炎抗性遗传改良提供了理论基础。Mastitis is one of the most common diseases with a negative economic impact on the dairy industry. We previously identified foursingle nucleotide polymorphism(SNPs)significantly associated withmastitis in TRAPPC9、CD4 and DAGT1.The aim of the study is to verify the correlation between SNPs and mastitis in an extended validation cohort of Chinese Holstein cattle. Wecollected blood samples from 895 Chinese Holstein cows and extracted DNA forSNP typing. To investigate the genetic effect of the SNPs on resistance to mastitis and milk production traits,used the results of SNP typing and DHI data in 2020, a general linear model was established. The association analysis showed that SNP1、SNP2、SNP3 and SNP4had significant effect on SCC or SCS in months(P<0.05).SNP1 and SNP4 had significant effect on 305-day milk yields in months(P<0.05).This study suggests that SNP1、SNP2、SNP3 and SNP4are related to mastitis resistance in Chinese Holstein cattle,and TRAPPC9、CD4 and DGAT1 could serve as genetic markers. The research offers a basis for the improvement of mastitis resistance in Chinese Holstein cattle.

关 键 词：中国荷斯坦牛 乳房炎 SNP 扩群验证 一般线性模型 

分 类 号：S823.2[农业科学—畜牧学]