Design and characterization of a bi-functional bybrid antibacterial peptide LLM against Pseudomonas aeruginosa  

作　　者：ZHANG Ze-jin YAO Hong-ji XUE Zhao-yi ZHAN Yu-tong GUO Ying-jie WU Meng LIN Ke-xin XIONG Jing-yan ZHANG Yong 

机构地区：[1]School of Basic Medicine and Life Science,Hainan Medical University,Haikou 571199,China [2]The First Clinical College of Hainan Medical University,Haikou 571199,China

出　　处：《Journal of Hainan Medical University》2022年第21期27-32,共6页海南医学院学报（英文版）

基　　金：supported by Scientific Research Project of Hainan Colleges and Universities(No.Hnky2019ZD-32);Innovation and Entrepreneurship Training Planning Project for College Students(No.S202011810018)。

摘　　要：Objective:To design a bifunctional antimicrobial peptide with high antibacterial activity and endotoxin neutralization against P.aeruginosa and explore its bactericidal properties.Methods:The neutralizing endotoxin peptides and antimicrobial peptide against P.aeruginosa were connected by a GGGS-linker to get a bi-functional bybrid peptide.Its structural parameter was tested by EMBOSS software.The minimum inhibitory concentration(MIC),minimum bactericidal concentration(MBC)and bactericidal kinetics against P.aeruginosa were determined.Its effect on endotoxin neutralization and hemolysis were also evaluated.Results:We designed and obtained a bybrid peptide LLM,which carried+8 positive charge with high activity against P.aeruginosa and neutralizing endotoxin.The MIC of LLM against P.aeruginosa CMCC10104 and P.aeruginosa ATCC 9027 were 2 and 4μmol/L,and MBC/MIC equal 1.LLM showed rapid anti-P.aeruginosa effects and significantly neutralized the endotoxin released,and not exhibited hemolysis as high as 115μmol/L(400μg/mL).Conclusion:The MICs of LLM against P.aeruginosa were 2~4μmol/L,it showed significant activity of anti-P.aeruginosa and neutralizing endotoxin,and could kill bacteria quickly,and did not show significant hemolytic under 115μmol/L.

关 键 词：Hybrid antimicrobial peptides Pseudomonas aeruginosa Endotoxin neutralization BACTERICIDAL HEMOLYTIC 

分 类 号：R91[医药卫生—药学]