甲酰基去氧胆酸修饰的Fe_(3)O_(4)纳米粒子的制备及其对土霉素负载的应用  

Preparation of Formyl Deoxycholic Acid Modified Fe_(3)O_(4) Nanoparticles and Their Application for Oxytetracycline Loading

作　　者：弓韬[1,2] 文朝朝 秦凯莉 赵莹珠 张宇奇 梁文婷 董川 GONG Tao;WEN Chao-Chao;QIN Kai-Li;ZAHO Ying-Zhu;ZHANG Yu-Qi;LIANG Wen-Ting;DONG Chuan(Department of Biochemistry and Molecular Biology,Shanxi Medical University,Taiyuan 030001,China;Institute of Environmental Science,Shanxi University,Taiyuan 030006,China)

机构地区：[1]山西医科大学基础医学院,太原030001 [2]山西大学环境科学研究所,太原030006

出　　处：《应用化学》2022年第12期1920-1926,共7页Chinese Journal of Applied Chemistry

基　　金：国家自然科学基金(No.21976113);山西省自然科学基金(No.202103021224240)资助。

摘　　要：成功构建了甲酰基去氧胆酸修饰的磁性Fe_(3)O_(4)纳米粒子(FDCA-MNPs),采用红外光谱和透射电子显微镜等手段研究了复合材料的结构、形貌、粒径以及磁性能。以制备的磁性纳米材料为药物载体,用荧光光谱法研究了FDCA-MNPs磁性纳米复合物对于土霉素(OTC)的载药和释药性能,并提出了可能的载药机理。考察了37℃的条件下,pH值在7和9时载体的药物负载和药物释放性能,发现药物的释放率在pH=9时(11.8%)高于pH=7(8.8%)。此外,在体外条件下对比了药物载体、土霉素和药物载体-土霉素复合物在不同浓度的条件下对大肠杆菌的抑菌效果,结果表明FDCA-MNPs本身不具有杀灭大肠杆菌的能力,与土霉素相比,负载有土霉素的纳米药物复合物在相同浓度条件下展现出了更好的灭菌能力。在实验条件下最小抑菌浓度为3μg/mL,此时抑菌率达到97.8%。证明磁性纳米载体与药物的协同作用可以有效地杀菌,从而减少抗生素的用量。Formyldeoxycholic acid modified Fe_(3)O_(4)nanoparticles(FDCA-MNPs)have been successfully constructed.The structure,morphology and magnetic properties of FDCA-MNPs are characterized by Fourier transform infrared spectroscopy(FT-IR),transmission electron microscopy(TEM)and vibrating sample magnetometer.Oxytetracycline(OTC)is used as a model drug to evaluate the drug loading and release behaviors of FDCA-MNPs.The maximum loading of OTC is 59. 7 mg/g,and the loading behavior follows Freundlich adsorption balance equations with multilayer adsorption.Furthermore,FDCA-MNPs@OTC shows a higher release rate at pH=9(11. 8%)than that of pH=7(8. 8%). The bacteriostatic activities of FDCA-MNPs,OTC,FDCA-MNPs@OTC against E. coli are compared in vitro. The results show that FDCA-MNPs have no inhibition-activity for E. coli,meanwhile,FDCA-MNPs@OTC displays better sterilization ability than OTC at the same concentration. The minimum antibacterial concentration is 3 μg/m L,and the antibacterial rate reaches 97. 8%. It is proved that the synergistic effect of drug carrier and drugs reveals good antibacterial properties and reduce the dosage of antibiotics.

关 键 词：Fe_(3)O_(4)纳米粒子 土霉素 药物释放 

分 类 号：O651[理学—分析化学]