补肾活血复方对慢性心力衰竭大鼠心室重构及心肌NF-κB蛋白的影响  

Effect of Bushen Huoxue Decoction on ventricular remodeling and myocardial NF-κB protein in rats with chronic heart failure

作　　者：许瑞 张艳[2] 何佳[3] 尹举 邹文聪 尹文浩 张伟[1] 刘贤娴 Xu Rui;Zhang Yan;He Jia;Yin Ju;Zou Wencong;Yin Wenhao;Zhang Wei;Liu Xianxian(Graduate School,Liaoning University of Traditional Chinese Medicine,Shenyang 110847,China;Specialist Clinic,the First Affiliated Hospital of Liaoning University of Traditional Chinese Medicine,Shenyang 110032,China;Post Doctoral Mobile Station,Liaoning University of Traditional Chinese Medicine,Shenyang 110847,China)

机构地区：[1]辽宁中医药大学研究生院,沈阳110847 [2]辽宁中医药大学附属医院专家门诊,沈阳110032 [3]辽宁中医药大学博士后流动站,沈阳110847

出　　处：《国际中医中药杂志》2022年第11期1253-1258,共6页International Journal of Traditional Chinese Medicine

基　　金：国家自然科学基金项目(82174241)。

摘　　要：目的探讨补肾活血复方对慢性心力衰竭(CHF)大鼠心室重构及心肌NF-κB蛋白表达的影响。方法将60只SD大鼠按随机数字表法分为假手术组15只和实验组45只。实验组大鼠通过结扎冠脉左前降支联合力竭式游泳、饥饿,建立CHF模型。将CHF模型大鼠分为模型组、补肾活血组及赖诺普利组。补肾活血组灌胃补肾活血中药汤剂15.75 g/(kg·d),赖诺普利组灌胃赖诺普利混悬液1.8 mg/(kg·d),假手术组和模型组灌胃等体积蒸馏水。给药4周后,观察大鼠一般精神状态情况,采用心脏彩超测定大鼠左室收缩末内径(LVIDs)及舒张末内径(LVIDd),并计算左心室射血分数(LVEF)与短轴缩短分数(LVFS),Western blot法检测大鼠心肌NF-κB蛋白表达,HE染色观察大鼠左心室肌细胞形态。结果与模型组比较,补肾活血组和赖诺普利组大鼠心肌纤维排列尚整齐,较少见胞核固缩,少量炎性细胞浸润。与模型组比较,补肾活血组大鼠LVIDd[(6.00±0.58)mm比(6.99±0.90)mm]、LVIDs[(3.96±0.51)mm、(5.14±0.57)mm]降低,LVEF[(54.48±6.75)%比(30.28±4.85)%]、LVFS[(33.86±4.27)%比(26.10±4.96)%]升高,心肌NF-κB[(1.06±0.10)比(1.58±0.29)]蛋白表达降低(P<0.05)。结论补肾活血复方可抵抗CHF大鼠心室重构,改善心功能,发挥治疗CHF的作用,可能与下调心肌NF-κB蛋白表达有关。Objective To explore the effect of Bushen Huoxue Decoction on ventricular remodeling and myocardial nuclear factor-kappaB(NF-κB)protein in rats with chronic heart failure.Methods 60 male SD rats were randomly divided into sham operation group(15 rats)and experimental group(45 rats).The rats of the experimental group was established CHF model by ligating the left anterior descending coronary artery combined with exhaustive swimming and starvation.Rats with chronic heart failure were randomly divided into model group,Bushen Huoxue group and lisinopril group.The Bushen Huoxue group was perfused with 15.75 g/(kg·d)Bushen Huoxue Decoction,the lisinopril group was perfused with 1.8 mg/(kg·d)of lisinopril suspension,and the sham operation group and model group were perfused with equal volume of distilled water.After 4 weeks of administration,the general mental state of rats was observed.The left ventricular internal systolic diameter(LVIDs)and internal diastolic diameter(LVIDd)were measured by cardiac color Doppler ultrasound,and the left ventricular ejection fraction(LVEF)and short axis shortening fraction(LVFS)were calculated.The expression of NF-κB protein in rat myocardium was detected by Western blot,and the morphology of left ventricular myocytes was observed by hematoxylin eosin staining.Results Compared with the model group,the myocardial fibers of rats in Bushen Huoxue group and lisinopril group were arranged orderly,with few pyknosis,a small amount of inflammatory cell infiltration.Compared with the model group,the levels of LVIDs[(6.00±0.58)mm vs.(6.99±0.90)mm]and LVIDd[(3.96±0.51)mm vs.(5.14±0.57)mm]significantly decreased,LVEF[(54.48±6.75)%vs.(30.28±4.85)%]and LVFS[(33.86±4.27)%vs.(26.10±4.96)%]significantly increased,as well as the expression of myocardial NF-κB(1.06±0.10 vs.1.58±0.29)protein significantly decreased(P<0.05).Conclusion Bushen Huoxue Decoction can resist ventricular remodeling,improve cardiac function and treat heart failure of CHF rats and the possible mechanism might be it c

关 键 词：心力衰竭 补肾活血复方 NF-ΚB 心室重构 大鼠 

分 类 号：R285.5[医药卫生—中药学]