A Novel Method to Improve the Physical Property and Biocompatibility of Decellularized Heart Valve Scaffold with Sericin and Polydopamine  

作　　者：Peng Bai Gangcheng Kong Weihua Qiao Yu Song Yixuan Wang Jiawei Shi Nan Zhang Chungeng Liu Chong Chu Tixiusi Xiong Ying Zhou Cuifen Lu Lin Wang Nianguo Dong 

机构地区：[1]Department of Cardiovascular Surgery,Union Hospital,Tongji Medical College,Huazhong University of Science and Technology,Wuhan 430032,China [2]Department of Hepatobiliary and Pancreatic Surgery,The First Affiliated Hospital,School of Medicine,Zhejiang University,Hangzhou 310003,China [3]Research Center for Tissue Engineering and Regenerative Medicine,Union Hospital,Tongji Medical College,Huazhong University of Science and Technology,Wuhan 430022,China [4]Department of Endocrinology,Union Hospital,Tongji Medical College,Huazhong University of Science and Technology,Wuhan 430032,China [5]Collaborative Innovation Center for Advanced Organic Chemical Materials Co-constructed by the Province and Ministry,Hubei University,Wuhan 430062,China [6]Department of Clinical Laboratory,Union Hospital,Tongji Medical College,Huazhong University of Science and Technology,Wuhan 430022,China

出　　处：《Journal of Bionic Engineering》2022年第4期1109-1123,共15页仿生工程学报（英文版）

基　　金：supported by the National Key Research and Development Program of China Stem Cell and Translational Research(2016YFA0101103);the National Natural Science Foundation of China(grant numbers 81930052,81901904,82000367,82001701).

摘　　要：Cardiac valve replacement is an effective method to treat valvular heart disease.Artificial valves used routinely in clinic still have defects.In our study,we explored a novel method to modify the performance of Decellularized Heart Valve(DHV)scaffold.The decellularized porcine aortic valve was prepared using sequential hydrophile and lipophile solubilization method.The sericin was extracted from silk fibroin-deficient silkworm cocoon by lithium bromide method.First,DHV was immersed in sericin solution to produce the sericin–DHV composite scaffold.Then,we modified the DHV by making a Polydopamine(PDA)coating on the DHV first and then binding the sericin.The physical properties and biological compatibility of our composite scaffold were assessed in vitro and in vivo.Sericin were successfully prepared,combined to DHV and improved its biocompatibility.PDA coating further promoted the combination of sericin on DHV and improved the physical properties of scaffolds.The decay rate of our modified valve scaffold was decreased in vivo and it showed good compatibility with blood.In conclusion,our modification improved the physical properties and biocompatibility of the valve scaffold.The combination of PDA and sericin promoted the recellularization of decellularized valves,showing great potential to be a novel artificial valve.

关 键 词：Decellularized heart valve SERICIN POLYDOPAMINE BIOCOMPATIBILITY 

分 类 号：S85[农业科学—兽医学]