关中奶山羊规模化胚胎移植技术研究  

Study on large-scale embryo transfer technology of Guanzhong dairy goats

作　　者：李德娴 王广 张永涛 付明哲[1] 陈晓强[1] 潘瑞[1] 邹家浩 李广[1] LI Dexian;WANG Guang;ZHANG Yongtao;FU Mingzhe;CHEN Xiaoqiang;PAN Rui;ZOU Jiahao;LI Guang(College of Animal Science and Technology,Northwest A&F University,Yangling 712100,China)

机构地区：[1]西北农林科技大学动物科技学院,陕西杨凌712100

出　　处：《黑龙江畜牧兽医》2022年第24期44-48,共5页Heilongjiang Animal Science And veterinary Medicine

基　　金：陕西省农业协同创新与推广联盟项目(LMZD202002);陕西省农业科技创新驱动项目(NYKJ-2021-ST-03)。

摘　　要：为了探究在不同月份采用不同超数排卵(以下简称超排)和胚胎移植处理对关中奶山羊规模化胚胎移植效果的影响,试验将248只高产供体关中奶山羊母羊分别于8,9,10月份分3批进行超排处理,每批又分为试验1组和试验2组,试验1组采用“前列腺素(PG)+孕酮栓(CIDR)+促卵泡素(FSH)+促黄体素释放激素A3(LHRH-A3)”的方式进行超排处理,试验2组在试验1组基础上添加孕马血清促性腺激素(PMSG)进行超排处理,供试母羊排卵后采用公羊自然配种,收集供体母羊胚胎并筛选有效胚胎进胚胎移植,试验期间测定平均黄体数、平均冲胚数、平均有效胚胎数、超排率;将1770只受体关中奶山羊母羊分别于8,9,10月份分3批进行胚胎移植,每批又分为试验A组和试验B组,试验A组采用腹腔镜胚胎移植手术进行胎移植,试验B组采用常规胚胎移植手术进行胚胎移植,胚胎移植后进行妊娠诊断,母羊产羔后记录产羔数,试验期间测定手术时间、手术效率、妊娠率、产羔率。结果表明:试验2组的平均黄体数、平均冲胚数和平均有效胚胎数均极显著高于试验1组(P<0.01),超排率高于试验1组,但差异不显著(P>0.05);8,9,10月份的平均黄体数、平均冲卵数、平均有效胚胎和超排率差异不显著(P>0.05),但均以9月份最高。试验A组的妊娠率和手术效率均极显著高于试验B组(P<0.01),手术时间极显著短于试验B组(P<0.01);9月份的妊娠率和产羔率最高。说明采用“PG+CIDR+FSH+LHRH-A3”的基础上添加“PMSG”的超排处理方法和腹腔镜胚胎移植手术于9月份进行胚胎移植的效果最好。In order to explore the effects of different superovulation and embryo transfer treatments on the large-scale embryo transfer of Guanzhong dairy goats in different months,a total of 248 high-yield donor Guanzhong dairy ewes were divided into three batches for superovula-tion treatment in August,September and October.Each batch was divided into test group 1 and test group 2.The test group 1 was superovulated with“prostaglandin(PG)+progesterone suppository(CIDR)+follicle stimulating hormone(FSH)+luteinizing hormone releasing hormone A3(LHRH-A3)”.On the basis of the test group 1,the test group 2 was added with“pregnant horse serum gonadotropin(PMSG)”for superovula-tion.After ovulationin,the test ewes and male sheep were used for natural mating.Donor ewe embryos were collected and selected for embryo transfer.During the experiment,the average number of corpus luteum,the average number of washed embryos,the average effective embryos and the superovulation rate were measured.A total of 1770 recipient Guanzhong dairy goats were divided into 3 batches for embryo transfer in August,September and October,and each batch was divided into test group A and test group B.The test group A was transferred by laparo-scope,and the test group B was transferred by conventional embryo surgery.Pregnancy diagnosis was conducted after embryo transfer.The number of lambs born after lambing was recorded.During the test,the operation time,the operation efficiency,the pregnancy rate and lambing rate were measured.The results showed that the average number of corpus luteum,the average number of washed embryos and the average num-ber of effective embryos in the test group 2 were extremely significantly higher than those in the test group 1(P<0.01);the superovulation rate was higher than that of the test group 1,but the difference was not significant(P>0.05).There was no significant difference in the average number of corpus luteum,the average number of washed eggs,the average effective embryos and the superovulation rate in August,Septe

关 键 词：关中奶山羊 腹腔镜 规模化 超数排卵 胚胎移植 孕马血清促性腺激素 促黄体素释放激素A3 

分 类 号：S826[农业科学—畜牧学]