圈养东北虎源猫杯状病毒的巢氏PCR检测及ORF2基因遗传进化分析  

Nested PCR detection and genetic evolutionary analysis of ORF2 gene fragments of Feline calicivirus originating from captive Northeast tigers

作　　者：周易 林立森 杨四权 赵亮[1] 徐海涛[2] 宫明[2] 李大鹏[2] 周晓禹[2] 王亚君[1] ZHOU Yi;LIN Lisen;YANG Siquan;ZHAO Liang;XU Haitao;GONG Ming;LI Dapeng;ZHOU Xiaoyu;WANG Yajun(College of Wildlife and Protected Area,Northeast Forestry University,Harbin 150040,China;Heilongjiang Northeast Tiger Park,Harbin 150028,China)

机构地区：[1]东北林业大学野生动物与自然保护地学院,哈尔滨150040 [2]黑龙江东北虎林园,哈尔滨150028

出　　处：《黑龙江畜牧兽医》2022年第24期80-84,共5页Heilongjiang Animal Science And veterinary Medicine

基　　金：国家级大学生创新创业训练计划项目(202110225004)。

摘　　要：为了解猫杯状病毒(Feline calicivirus,FCV)在圈养东北虎中的流行情况,试验在黑龙江省海林市横道河子东北虎林园与哈尔滨市东北虎林园共采集东北虎血液样本40份,采用巢氏PCR对FCV进行检测,并对阳性PCR产物进行测序和遗传进化分析。结果表明:40份样品中共有4份检测为FCV阳性,阳性率为10%。4株测序毒株分别命名为HB-1516、HD-068、HD-079、HD101,与FCV参考株的核苷酸相似性为76.6%~88.3%;4株测序毒株处在同一分支,且与猫源FCV亲缘关系最近,但与常用疫苗株(F4、F9、F65、FCV2024株)处在不同分支。说明可能存在FCV由猫传染虎的情况,提示目前使用的疫苗不能起到完全保护作用。In order to understand the prevalence of Feline calicivirus(FCV)in captive Northeast tigers,in this experiments a total of 40 blood samples of Northeast tigers were collected from Hengdaohezi Northeast Tiger Park in Hailin City and Northeast Tiger Park in Harbin City,Heilongjiang Province.FCV was detected by nested PCR,and positive PCR products were sequenced and analyzed for genetic evolution.The results showed that a total of 4 out of 40 samples were tested positive for FCV,with a positive rate of 10%.Four sequencing isolates were named HB-1516,HD-068,HD-079,and HD101,respectively.The nucleotide similarities between the four sequencing isolates and the FCV reference strains ranged from 76.6% to 88.3%;the four sequencing isolates were in the same branch and were most closely related to the cat-derived FCV,but were in different branches with the commonly used vaccine strains(F4,F9,F65,2024 strain).The results suggested that there might be FCV transmission from cats to tigers,suggesting that the vaccine currently in use did not provide complete protection.

关 键 词：东北虎 猫杯状病毒 ORF2基因 分子流行病学 巢氏PCR 

分 类 号：S852.65[农业科学—基础兽医学] Q78[农业科学—兽医学]