复齿鼯鼠细菌混合感染性肺炎的诊断与治疗  

Bacterial mixed infection pneumonia in compound-toothed flying squirrels

作　　者：祁桂祥 戴鹏秀 徐浩杰 张欣珂[1] QI Guixiang;DAI Pengxiu;XU Haojie;ZHANG Xinke(College of Veterinary Medicine,Northwest Agriculture and Forestry University,Yangling 712100,China)

机构地区：[1]西北农林科技大学动物医学院,陕西杨凌712100

出　　处：《黑龙江畜牧兽医》2022年第24期92-95,138,139,共6页Heilongjiang Animal Science And veterinary Medicine

摘　　要：2021年8月初,陕西省蓝田县某复齿鼯鼠养殖场内复齿鼯鼠突然发病,呕咳不止,口鼻流黏液样分泌物,不停翻滚抽搐,总死亡率为21%,传染性强,给养殖户造成了巨大的经济损失。为了对该养殖场复齿鼯鼠所患疾病进行诊断与治疗,试验通过剖检死亡复齿鼯鼠,初步确定病变部位,并采集病变组织器官病料;将采集的病料接种于普通琼脂培养基和血琼脂培养基,分离纯化后用分子生物学方法鉴定病原菌种类,对分离到的病原菌进行药敏试验,并根据病原菌鉴定结果与药敏试验结果进行治疗。结果表明:剖检可见复齿鼯鼠肺脏淤血,呼吸系统受损;细菌分离培养可见灰白色、光滑、湿润的圆形菌落;PCR产物测序结果显示,分离菌株与鼠流感嗜血杆菌核苷酸相似性为99.00%,与松鼠葡萄球菌核苷酸相似性为98.21%;且两种病原菌均对左氧氟沙星敏感,选择左氧氟沙星治疗后,复齿鼯鼠传染性肺炎得到有效控制,没有继续出现死亡病例。说明该场复齿鼯鼠感染了由鼠流感嗜血杆菌和松鼠葡萄球菌引起的细菌混合感染性肺炎,左氧氟沙星可用于该复齿鼯鼠养殖场的临床防治。In early August 2021,flying squirrels in a compound-toothed flying squirrel farm in Lantian County,Shaanxi Province,suddenly developed the disease of vomiting and coughing,with mucus-like secretions from the mouth and nose,non-stop tumbling and convulsin,with a total mortality rate of 21%and strong contagiousness,which caused huge economic losses to the farmers.In order to diagnose and treat the disease suffered by the compound-toothed flying squirrels in this farm,the experiment was conducted by dissecting the dead compound-toothed flying squirrels,initially identifying the lesion sites and collecting diseased tissue and organ materials.The collected disease material was inoculated in normal agar medium and blood agar medium,and the pathogenic species were identified by molecular biology after isolation and purification,and the pathogenic bacteria isolated were tested for drug sensitivity and treated according to pathogen identification results and drug sensitivity test results.The results showed that the lungs of the compound-toothed flying squirrels were stasis and the respiratory system was damaged on autopsy;the bacterial isolation culture showed gray-white,smooth,moist,round colonies.The PCR product sequencing results showed that the isolates had 99.00% nucleotide similarity with mouse Haemophilus influenzae and 98.21% nucleotide similarity with Mammaliicoccus sciuri;and both pathogens were susceptible to levofloxacin.After the selection of levofloxacin treatment,infectious pneumonia in compound-toothed flying squirrels was effectively controlled and no further mortality cases occurred.The results suggested that the compound-toothed flying squirrels in this farm were infected with bacterial mixed infection pneumonia caused by Haemophilus influenzae and Mammaliicoccus sciuri,and levofloxacin could be used for clinical control in this compound-toothed flying squirrel farm.

关 键 词：复齿鼯鼠 病原菌分离 药敏试验 细菌性肺炎 诊断 治疗 

分 类 号：S856.3[农业科学—临床兽医学]